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GrainGenes Reference Report: EJP-107-661

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Reference	EJP-107-661
Title	Molecular identification of Oat mosaic virus as a Bymovirus
Journal	European Journal of Plant Pathology
Year	2001
Volume	107
Pages	661-666
Author	Monger WA Clover GRG Foster GD
Abstract	Summary: A partial sequence of Oat mosaic virus (OMV) has been obtained for four isolates of the virus from four European countries. This represents the first available sequence data for this important disease of winter-sown oats. The longest clone of 1699 nucleotides was obtained from infected English oats using a degenerate primer, designed to members of the Potyviridae family. Alignment of the predicted amino acid sequence with members of the Potyviridae showed closest identity with viruses of the Bymovirus genus. The predicted amino acid sequence has one open reading frame corresponding to part of the NIb and capsid protein, with a 3' untranslated region of 351 nucleotides, followed by a poly(A) tail. PCR primers were designed to the coat protein and NIb gene of members of the Bymovirus genus and used to obtain partial sequences of 1441 nucleotides at the 3' end of infected oats from both Wales and France. A specific primer set designed to the English isolate was used to generate a product of 701 nucleotides from OMV-infected oat leaves from Ireland. All four isolates are highly conserved at the amino acid level
Keyword	[ Hide all but 1 of 38 ] acid alignment amino acid amino acid sequence bymovirus capsid protein clone coat coat protein degenerate primer disease family france gene genus isolate member molecular identification mosaic-virus nucleotide oat oat leaves omv open reading frame pcr pcr primers poly(a) tail potyviridae primer protein reading frame region rt-pcr sequence sequence data untranslated region virus wales

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