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GrainGenes Reference Report: EJP-107-285

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Reference	EJP-107-285
Title	Genotypic diversity of the wheat leaf blotch pathogen Mycosphaerella graminicola (anamorph) Septoria tritici in Germany
Journal	European Journal of Plant Pathology
Year	2001
Volume	107
Pages	285-290
Author	Schnieder F Koch G Jung C Verreet JA
Abstract	Summary: The population structure and genotypic diversity of Mycosphaerella graminicola from six natural field populations in Germany were studied with molecular markers. To reveal the potential effects of plant host resistance on the pathogen population, hierarchical samples were taken from susceptible and resistant cultivars. A total of 203 single spore isolates was subjected to molecular marker analysis using the amplified fragment length polymorphism technique (AFLP). Among the 203 isolates analyzed, 142 different multilocus haplotypes (MLH) were identified revealing a high degree of genotypic diversity of the M. graminicola population. On average, a F(ST) value of 0.04 was found, indicating a low genetic differentiation with only 4% of the genetic variation between the local populations but leaving 96% of the genetic variation within the populations. According to the low F(ST) value, a high migration rate of Nm approximately equal to 12 was found. The observed high within-population diversity, and the significant migration between populations, prevented genetic isolation and differentiation of putative geographically separated populations. Furthermore, plant host resistance had no obvious effect on the population structure and diversity of M. graminicola. Genotypic variability can be attributed to sexual recombination which appears to have a considerably larger influence on the population structure. Gene flow on this scale could have significant implications for plant breeding and fungicide spraying programmes
Keyword	[ Hide all but 1 of 55 ] aflp amplified fragment length polymorphism blotch breeding cultivar differentiation distance diversity dna fingerprinting field field populations flow fragment length polymorphism fungi gene gene flow genetic genetic differentiation genetic variation genotypic diversity germany graminicola haplotype host host-resistance isolate isolation leaf blotch length polymorphism marker migration molecular marker multilocus haplotypes mycosphaerella graminicola pathogen pathogen population pcr plant breeding plant science polymorphism population population genetics population structure rate recombination resistance resistant scale septoria tritici sexual recombination single structure tritici variability wheat leaves

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