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GrainGenes Reference Report: EJB-269-5377

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Reference	EJB-269-5377
Title	Barley alpha-amylase Met53 situated at the high-affinity subsite-2 belongs to a substrate binding motif in the beta ->alpha loop 2 of the catalytic (beta/alpha)(8)-barrel and is critical for activity and substrate specificity
Journal	European Journal of Biochemistry
Year	2002
Volume	269
Pages	5377-5390
Author	Mori H Bak-Jensen KS Svensson B
External Databases	Pubmed: 12423336
Keyword	[ Hide all but 1 of 28 ] action pattern alpha-amylase amylose angstrom resolution barley binding subsite engineering crystal structure cyclodextrin glycosyltransferase family forming exo-amylase glycoside hydrolase family 13 linkage molecular structure mutant oligosaccharide hydrolysis pattern plant alpha-amylases residue secreted proteins sequence site-directed mutagenesis specificity starch substrate transglycosylation activity wild x-ray structure yeast pichia pastoris

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