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GrainGenes Reference Report: EJB-268-6545

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Reference	EJB-268-6545
Title	Modulation of activity and substrate binding modes by mutation of single and double subsites+1/+2 and-5/-6 of barley alpha-amylase 1
Journal	European Journal of Biochemistry
Year	2001
Volume	268
Pages	6545-6558
Author	Mori H [ Show all 7 ]
External Databases	Pubmed: 11737209
Keyword	[ Hide all but 1 of 56 ] (beta/alpha)(8) barrel 2.2-angstrom resolution acid action pattern alpha alpha-amylase alpha-amylase-1 amino acid amino acid substitution amylase amylose angstrom resolution bacillus subtilis barley barley alpha-amylase beta binding biochemistry biophysics branched malto-oligosaccharide chain cleavage crystal crystal structure end family glucose glycogen glycoside hydrolase family 13 impact inhibitor isozyme k-m malto-oligosaccharide modulation mutant mutation properties purification ratios release secreted proteins single site-directed mutagenesis specificity starch structure subsite substitution substrate substrate binding substrate specificity substrates sulfur wild-type yeast pichia pastoris

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