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GrainGenes Reference Report: DNA-16-81

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Reference	DNA-16-81
Title	Development of 5006 full-length CDNAs in barley: a tool for accessing cereal genomics resources.
Journal	DNA Research
Year	2009
Volume	16
Pages	81-89
Author	Sato K [ Show all 9 ]
Abstract	A collection of 5006 full-length (FL) cDNA sequences was developed in barley. Fifteen mRNA samples from various organs and treatments were pooled to develop a cDNA library using the CAP trapper method. More than 60% of the clones were confirmed to have complete coding sequences, based on comparison with rice amino acid and UniProt sequences. Blastn homologies (E<1E-5) to rice genes and Arabidopsis genes were 89 and 47%, respectively. Of the 5028 possible amino acid sequences derived from the 5006 FLcDNAs, 4032 (80.2%) were classified into 1678 GreenPhyl multigenic families. There were 555 cDNAs showing low homology to both rice and Arabidopsis. Gene ontology annotation by InterProScan indicated that many of these cDNAs (71%) have no known molecular functions and may be unique to barley. The cDNAs showed high homology to Barley 1 GeneChip oligo probes (81%) and the wheat gene index (84%). The high homology between FLcDNAs (27%) and mapped barley expressed sequence tag enabled assigning linkage map positions to 151-233 FLcDNAs on each of the seven barley chromosomes. These comprehensive barley FLcDNAs provide strong platform to connect pre-existing genomic and genetic resources and accelerate gene identification and genome analysis in barley and related species. Sequence data from this article have been deposited with the DDBJ/EMBL/GenBank Data Libraries under accession nos AK248134-AK253139. The online database with annotation is available at http://www.shigen.nig.ac.jp/barley/.
External Databases	http://dx.doi.org/10.1093/dnares/dsn034
Locus	AK250858.1 [ Show all 4991 ]
Probe	[ Hide all but 1 of 4991 ] FLbaf100a02 FLbaf100a04 FLbaf100a05 FLbaf100b05 FLbaf100b21 FLbaf100c06 FLbaf100c08 FLbaf100d03 FLbaf100d08 FLbaf100e02 FLbaf100e03 FLbaf100e06 FLbaf100e15 FLbaf100e22 FLbaf100f01 FLbaf100f10 FLbaf100f13 FLbaf100g18 FLbaf100i02 FLbaf100i22 FLbaf100j05 FLbaf100j08 FLbaf100j17 FLbaf100j21 FLbaf100k05 FLbaf100k11 FLbaf100k12 FLbaf100k19 FLbaf100l04 FLbaf100l17 FLbaf100l20 FLbaf100l24 FLbaf100m01 FLbaf100n14 FLbaf100o03 FLbaf100o05 FLbaf100o18 FLbaf100p04 FLbaf100p19 FLbaf101a03 FLbaf101a15 FLbaf101a21 FLbaf101b07 FLbaf101c04 FLbaf101d01 FLbaf101e05 FLbaf101e19 FLbaf101f05 FLbaf101f24 FLbaf101g03 FLbaf101g05 FLbaf101h20 FLbaf101h24 FLbaf101i01 FLbaf101i10 FLbaf101k22 FLbaf101l12 FLbaf101m05 FLbaf101n03 FLbaf101n05 FLbaf101n06 FLbaf101n11 FLbaf101o12 FLbaf102a05 FLbaf102a14 FLbaf102a21 FLbaf102b17 FLbaf102c12 FLbaf102c19 FLbaf102d23 FLbaf102e02 FLbaf102e05 FLbaf102e15 FLbaf102g14 FLbaf102i21 FLbaf102j05 FLbaf102j14 FLbaf102j23 FLbaf102k03 FLbaf102k14 FLbaf102l03 FLbaf102l04 FLbaf102m02 FLbaf102m03 FLbaf102n14 FLbaf102n16 FLbaf102n18 FLbaf102n23 FLbaf102o07 FLbaf102p13 FLbaf103b01 FLbaf103b19 FLbaf103c03 FLbaf103d03 FLbaf103d06 FLbaf103d16 FLbaf103f22 FLbaf103g10 FLbaf103i10 FLbaf103i12 FLbaf103j17 FLbaf103k23 FLbaf103m08 FLbaf103n03 FLbaf103n06 FLbaf103o07 FLbaf103o08 FLbaf103o09 FLbaf103p23 FLbaf104a09 FLbaf104b09 FLbaf104b11 FLbaf104c07 FLbaf104c16 FLbaf104c19 FLbaf104c24 FLbaf104d03 FLbaf104d23 FLbaf104e02 FLbaf104e11 FLbaf104e19 FLbaf104f13 FLbaf104g20 FLbaf104g23 FLbaf104h05 FLbaf104i05 FLbaf104i23 FLbaf104j02 FLbaf104j18 FLbaf104k09 FLbaf104k14 FLbaf104l13 FLbaf104l16 FLbaf104m15 FLbaf104n05 FLbaf104n16 FLbaf104n17 FLbaf104o16 FLbaf105b19 FLbaf105b21 FLbaf105c09 FLbaf105c21 FLbaf105d17 FLbaf105d18 FLbaf105d21 FLbaf105e02 FLbaf105e08 FLbaf105e13 FLbaf105e18 FLbaf105e20 FLbaf105f23 FLbaf105h14 FLbaf105h20 FLbaf105h24 FLbaf105i02 FLbaf105i16 FLbaf105k18 FLbaf105k19 FLbaf105l15 FLbaf105l21 FLbaf105n06 FLbaf105n16 FLbaf105o01 FLbaf105o12 FLbaf105o15 FLbaf105p04 FLbaf105p05 FLbaf106a04 FLbaf106a20 FLbaf106b04 FLbaf106b22 FLbaf106c06 FLbaf106c13 FLbaf106c19 FLbaf106d18 FLbaf106e21 FLbaf106f03 FLbaf106f05 FLbaf106f06 FLbaf106f17 FLbaf106g06 FLbaf106g10 FLbaf106h07 FLbaf106i07 FLbaf106i08 FLbaf106j02 FLbaf106j08 FLbaf106k06 FLbaf106l09 FLbaf106m04 FLbaf106m14 FLbaf106m22 FLbaf106n02 FLbaf106n06 FLbaf106o11 FLbaf106p06 FLbaf106p21 FLbaf107a08 FLbaf107a24 FLbaf107b02 FLbaf107b03 FLbaf107b10 FLbaf107b17 FLbaf107c21 FLbaf107d05 FLbaf107d20 FLbaf107e06 FLbaf107e07 FLbaf107e14 FLbaf107f11 FLbaf107g20 FLbaf107h16 FLbaf107i24 FLbaf107j09 FLbaf107j17 FLbaf107k18 FLbaf107k22 FLbaf107l01 FLbaf107m01 FLbaf107n02 FLbaf107n19 FLbaf107o01 FLbaf107o06 FLbaf107o12 FLbaf107p09 FLbaf108a03 FLbaf108a10 FLbaf108a12 FLbaf108a17 FLbaf108c07 FLbaf108c11 FLbaf108c20 FLbaf108d15 FLbaf108d19 FLbaf108d20 FLbaf108e15 FLbaf108f14 FLbaf108f15 FLbaf108g03 FLbaf108g11 FLbaf108g12 FLbaf108g19 FLbaf108g21 FLbaf108h11 FLbaf108h14 FLbaf108h18 FLbaf108i19 FLbaf108i21 FLbaf108j06 FLbaf108k03 FLbaf108k05 FLbaf108k17 FLbaf108k24 FLbaf108l03 FLbaf108l07 FLbaf108l19 FLbaf108l22 FLbaf108n07 FLbaf108n08 FLbaf108n17 FLbaf108n20 FLbaf108n23 FLbaf108o05 FLbaf108p03 FLbaf108p05 FLbaf108p15 FLbaf109a09 FLbaf109a20 FLbaf109b17 FLbaf109d12 FLbaf109e18 FLbaf109g04 FLbaf109g06 FLbaf109g12 FLbaf109h08 FLbaf109h21 FLbaf109h24 FLbaf109i01 FLbaf109j09 FLbaf109j16 FLbaf109k10 FLbaf109k12 FLbaf109k13 FLbaf109l05 FLbaf109l16 FLbaf109l18 FLbaf109m22 FLbaf109n23 FLbaf109p16 FLbaf10b23 FLbaf10c10 FLbaf10c21 FLbaf10c23 FLbaf10d05 FLbaf10e14 FLbaf10e24 FLbaf10f01 FLbaf10g14 FLbaf10g16 FLbaf10h02 FLbaf10h22 FLbaf10i03 FLbaf10i21 FLbaf10j11 FLbaf10j16 FLbaf10j24 FLbaf10k02 FLbaf10k11 FLbaf10m05 FLbaf10m12 FLbaf10m16 FLbaf10n06 FLbaf10n08 FLbaf10n10 FLbaf10n20 FLbaf10n21 FLbaf10o13 FLbaf10o20 FLbaf10p09 FLbaf10p23 FLbaf110a14 FLbaf110a21 FLbaf110c05 FLbaf110c15 FLbaf110d04 FLbaf110d20 FLbaf110f02 FLbaf110g08 FLbaf110g24 FLbaf110j03 FLbaf110j10 FLbaf110k03 FLbaf110k09 FLbaf110k10 FLbaf110k14 FLbaf110l01 FLbaf110n02 FLbaf110n03 FLbaf110n18 FLbaf110o02 FLbaf110o14 FLbaf110o17 FLbaf110o20 FLbaf110o21 FLbaf111a11 FLbaf111b01 FLbaf111b06 FLbaf111b11 FLbaf111b13 FLbaf111b15 FLbaf111b24 FLbaf111c02 FLbaf111c09 FLbaf111c14 FLbaf111d06 FLbaf111d10 FLbaf111d20 FLbaf111d23 FLbaf111e07 FLbaf111e11 FLbaf111f19 FLbaf111g03 FLbaf111g10 FLbaf111g13 FLbaf111g21 FLbaf111h03 FLbaf111h08 FLbaf111h23 FLbaf111i02 FLbaf111i18 FLbaf111i19 FLbaf111j01 FLbaf111j11 FLbaf111j21 FLbaf111k14 FLbaf111k16 FLbaf111k21 FLbaf111l04 FLbaf111l08 FLbaf111l10 FLbaf111l21 FLbaf111m12 FLbaf111m13 FLbaf111m17 FLbaf111m18 FLbaf111m19 FLbaf111m21 FLbaf111n03 FLbaf111n05 FLbaf111n06 FLbaf111p06 FLbaf111p17 FLbaf111p24 FLbaf112b05 FLbaf112b12 FLbaf112c03 FLbaf112c09 FLbaf112c18 FLbaf112c24 FLbaf112d05 FLbaf112d07 FLbaf112d24 FLbaf112f15 FLbaf112h24 FLbaf112i05 FLbaf112i07 FLbaf112j09 FLbaf112j19 FLbaf112k03 FLbaf112l04 FLbaf112l08 FLbaf112l09 FLbaf112m07 FLbaf112m23 FLbaf112m24 FLbaf112n03 FLbaf112o04 FLbaf112o19 FLbaf112p01 FLbaf112p12 FLbaf113c04 FLbaf113d17 FLbaf113d20 FLbaf113e01 FLbaf113e04 FLbaf113e06 FLbaf113e21 FLbaf113e22 FLbaf113f21 FLbaf113h14 FLbaf113h22 FLbaf113i04 FLbaf113i14 FLbaf113j02 FLbaf113j06 FLbaf113j09 FLbaf113k13 FLbaf113l02 FLbaf113m21 FLbaf113m22 FLbaf113m23 FLbaf113n01 FLbaf113n11 FLbaf113n17 FLbaf113n22 FLbaf113o01 FLbaf113o03 FLbaf113p10 FLbaf113p19 FLbaf114b04 FLbaf114c04 FLbaf114c21 FLbaf114c23 FLbaf114d13 FLbaf114e02 FLbaf114e18 FLbaf114f20 FLbaf114g03 FLbaf114h02 FLbaf114i23 FLbaf114j09 FLbaf114j19 FLbaf114k06 FLbaf114k14 FLbaf114k16 FLbaf114l11 FLbaf114m12 FLbaf114n19 FLbaf114o02 FLbaf114o17 FLbaf115a07 FLbaf115a15 FLbaf115a19 FLbaf115b02 FLbaf115b04 FLbaf115c16 FLbaf115d02 FLbaf115d04 FLbaf115d07 FLbaf115d11 FLbaf115e03 FLbaf115e23 FLbaf115f13 FLbaf115h06 FLbaf115i02 FLbaf115i06 FLbaf115i14 FLbaf115i16 FLbaf115j11 FLbaf115j14 FLbaf115k03 FLbaf115k07 FLbaf115k20 FLbaf115l11 FLbaf115l18 FLbaf115m08 FLbaf115m13 FLbaf115n09 FLbaf115n24 FLbaf115o13 FLbaf115p07 FLbaf115p17 FLbaf115p19 FLbaf116b02 FLbaf116b12 FLbaf116c05 FLbaf116c16 FLbaf116c19 FLbaf116c23 FLbaf116g07 FLbaf116g11 FLbaf116g12 FLbaf116g19 FLbaf116h04 FLbaf116h05 FLbaf116h06 FLbaf116h24 FLbaf116j07 FLbaf116k01 FLbaf116k05 FLbaf116k09 FLbaf116k20 FLbaf116k22 FLbaf116m05 FLbaf116n16 FLbaf116n19 FLbaf116n20 FLbaf116n24 FLbaf116o06 FLbaf116p22 FLbaf116p23 FLbaf117b22 FLbaf117c22 FLbaf117c24 FLbaf117d02 FLbaf117d03 FLbaf117d04 FLbaf117d17 FLbaf117d20 FLbaf117e02 FLbaf117e03 FLbaf117f01 FLbaf117h13 FLbaf117h16 FLbaf117i11 FLbaf117i14 FLbaf117i15 FLbaf117j10 FLbaf117k09 FLbaf117k21 FLbaf117k24 FLbaf117l08 FLbaf117m06 FLbaf117m10 FLbaf117n08 FLbaf117n12 FLbaf117o12 FLbaf117o21 FLbaf117p03 FLbaf118a06 FLbaf118a15 FLbaf118b03 FLbaf118b19 FLbaf118d06 FLbaf118g07 FLbaf118g11 FLbaf118g22 FLbaf118h15 FLbaf118i17 FLbaf118j14 FLbaf118k14 FLbaf118k15 FLbaf118k16 FLbaf118m08 FLbaf118m19 FLbaf118n12 FLbaf118o06 FLbaf118p04 FLbaf119a19 FLbaf119a22 FLbaf119b09 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FLbaf127n06 FLbaf127o03 FLbaf127o07 FLbaf127o16 FLbaf127o19 FLbaf128a10 FLbaf128b06 FLbaf128c16 FLbaf128d15 FLbaf128e15 FLbaf128e19 FLbaf128e24 FLbaf128f05 FLbaf128g02 FLbaf128g07 FLbaf128g16 FLbaf128g18 FLbaf128h04 FLbaf128i09 FLbaf128j07 FLbaf128j19 FLbaf128j22 FLbaf128k13 FLbaf128l23 FLbaf128m02 FLbaf128n05 FLbaf128n06 FLbaf128n07 FLbaf128n16 FLbaf128n19 FLbaf128o05 FLbaf128o10 FLbaf128o12 FLbaf128o18 FLbaf128o24 FLbaf128p14 FLbaf128p17 FLbaf129a18 FLbaf129a20 FLbaf129b04 FLbaf129b06 FLbaf129b13 FLbaf129b18 FLbaf129c12 FLbaf129d05 FLbaf129d11 FLbaf129e19 FLbaf129e23 FLbaf129f04 FLbaf129f05 FLbaf129f09 FLbaf129g09 FLbaf129g14 FLbaf129h01 FLbaf129h07 FLbaf129h11 FLbaf129h12 FLbaf129h14 FLbaf129i03 FLbaf129i08 FLbaf129i10 FLbaf129j16 FLbaf129j21 FLbaf129k13 FLbaf129k20 FLbaf129k22 FLbaf129k23 FLbaf129l09 FLbaf129l17 FLbaf129m10 FLbaf129m20 FLbaf129n07 FLbaf129n18 FLbaf129o04 FLbaf129o09 FLbaf129o24 FLbaf12a06 FLbaf12c01 FLbaf12c02 FLbaf12d15 FLbaf12d18 FLbaf12e07 FLbaf12f04 FLbaf12f15 FLbaf12f20 FLbaf12g05 FLbaf12g06 FLbaf12g17 FLbaf12g24 FLbaf12h07 FLbaf12h09 FLbaf12h12 FLbaf12h23 FLbaf12j07 FLbaf12j20 FLbaf12k03 FLbaf12l15 FLbaf12m08 FLbaf12m24 FLbaf12o11 FLbaf12o13 FLbaf12o17 FLbaf12o18 FLbaf12p04 FLbaf12p08 FLbaf12p19 FLbaf130a05 FLbaf130b05 FLbaf130b18 FLbaf130c03 FLbaf130c18 FLbaf130d01 FLbaf130d11 FLbaf130d12 FLbaf130d24 FLbaf130e05 FLbaf130h06 FLbaf130h12 FLbaf130h24 FLbaf130j09 FLbaf130j12 FLbaf130j23 FLbaf130k12 FLbaf130k19 FLbaf130m20 FLbaf130n04 FLbaf130n05 FLbaf130n10 FLbaf130n16 FLbaf130n19 FLbaf130n23 FLbaf130o03 FLbaf130o13 FLbaf130o24 FLbaf130p13 FLbaf130p19 FLbaf131a18 FLbaf131a21 FLbaf131b19 FLbaf131c08 FLbaf131c12 FLbaf131c17 FLbaf131d08 FLbaf131d11 FLbaf131e04 FLbaf131e11 FLbaf131e13 FLbaf131e16 FLbaf131f17 FLbaf131f19 FLbaf131g20 FLbaf131g21 FLbaf131g24 FLbaf131h08 FLbaf131h10 FLbaf131h20 FLbaf131i21 FLbaf131j06 FLbaf131j09 FLbaf131j15 FLbaf131k14 FLbaf131l15 FLbaf131m24 FLbaf131n08 FLbaf131n14 FLbaf131n18 FLbaf131n23 FLbaf131p01 FLbaf131p06 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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.