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GrainGenes Reference Report: CUM-42-160

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Reference	CUM-42-160
Title	Effect of genomic location on horizontal transfer of a recombinant gene cassette between Pseudomonas strains in the rhizosphere and spermosphere of barley seedlings
Journal	Current Microbiology
Year	2001
Volume	42
Pages	160-167
Author	Sengelov G Kristensen KJ Sorensen AH Kroer N Sorensen SJ
Abstract	Summary: The use of genetically engineered bacteria in natural environments constitutes a risk of transfer of recombinant DNA to the indigenous bacteria. However, chromosomal genes are believed to be less likely to transfer than genes on mobilizable and conjugative plasmids. To study this assumption, horizontal transfer of a recombinant gene cassette inserted into the chromosome of a Pseudomonas stutzeri strain, into a mobilizable plasmid (pAGM42), and into a conjugative plasmid (pKJK5) isolated from barley rhizosphere was investigated. Horizontal transfer efficiencies of the gene cassette inserted into a conjugative plasmid was 8.20 X 10(-3) transconjugants/(donors X recipients)1/2 in the rhizosphere and 4.57 X 10(-2) transconjugants/(donors X recipients)1/2 in the spermosphere. Mobilization of the plasmid pAGM42 by the plasmids RP4 and pKJK5 was also detected at high levels in the microcosms, transfer efficiencies were up to 4.36 X 10(-3) transconjugants/(donors X recipients)1/2. Transfer of chromosomal encoded genes could not be detected in the microcosms by conjugation or transformation. However, transformation did occur by using the same bacterial strains under laboratory conditions. The rhizosphere and especially the spermosphere thus proved to be hot spot environments providing favorable conditions for gene transfer by mobilization and conjugation, but these environments did not support transformation at a detectable level
Keyword	[ Hide all but 1 of 27 ] aquatic environments bacteria barley barley rhizosphere barley seedlings chromosomal insertion chromosome conjugation dna environment gene gene transfer high-frequency microcosms natural environment natural transformation plasmid plasmids pseudomonas recombinant dna rhizosphere risk seedlings soil stutzeri transfer transformation

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