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GrainGenes Reference Report: CUG-25-456

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Reference	CUG-25-456
Title	Mitochondrial DNA variability detected in a single wheat regenerant involves a rare recombination event across a short repeat
Journal	Current Genetics
Year	1994
Volume	25
Pages	456-464
Author	Hartmann C [ Show all 9 ]
Abstract	The mitochondrial genome of the selfed progeny of a plant regenerated from long-term somatic tissue culture displays specific structural rearrangements characterized by the appearance of novel restriction fragments A mitochondrial DNA library was constructed from this selfed progeny in the SalI site of cosmid pHC79 and the novel fragments were subsequently studied. They were shown to arise from reciprocal recombination events involving DNA sequences present in the parental plant. The regions of recombination were sequenced and the nucleotide sequences were aligned with those of the presumptive parental fragments. We characterized an imperfect short repeated DNA sequence, 242 bp long, within which a 7-bp DNA repeat could act as a region of recombination. The use of PCR technology allowed us to show that these fragments were present in both parental plants and tissue cultures as low-abundance sequence arrangements.
Keyword	[ Hide all but 1 of 10 ] genetic variation mitochondrial dna mitochondrial genetics nucleotide sequence reciprocal-recombination recombination repetitive dna somaclonal-variation tissue culture triticum aestivum

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