GrainGenes Reference Report: CRS-44-1839
Reference
CRS-44-1839
Title
Effects of growth regulators on in vitro plant regeneration in durum wheat
Journal
Crop Science
Year
2004
Volume
44
Pages
1839-1846
Author
Satyavathi VV Jauhar PP Elias EM Rao MB
Abstract
Work on improvement of durum wheat (Triticum turgidum L.) using tools of biotechnology is limited. Development of a reliable in vitro plant regeneration procedure for this important cereal is a prerequisite for its improvement by genetic transformation. Here, we report the effects of three growth regulators (GRs), 2,4-D (2,4-dichlorophenoxyacetic acid), picloram (4-amino-3,5,6-trichloropicolinic acid), and dicamba (3,6-dichloro-o-anisic acid), on callus induction and plant regeneration from scutellum cultures of four commercial durum cultivars: Ben, Maier, Munich, and Lebsock. Callus induction was obtained from isolated scutella cultured on modified Murashige and Skoog (MS) basal medium. After 4 wk of callus induction, all calli were plated on MS basal medium for regeneration. The regenerated plantlets were fertile, maintained the normal chromosome number (2n=4x=28) and structure as revealed by fluorescent genomic in situ hybridization (fl-GISH), and showed no apparent somaclonal variation. Genotype and callus induction medium played a dominant role in plantlet regeneration. Dicamba proved the best GR for inducing compact callus and also gave the highest proportion (0.16) of regenerated plants across the four cultivars. Overall, Maier gave the highest proportion (0.27) of plantlet regeneration when dicamba at 2.0 mg L-1 concentration was used for initial callus induction. These results will facilitate genetic transformation work with durum wheat
Keyword
[ Hide all but 1 of 12 ] 2,4-d callus chromosome chromosome number cultivar dicamba genetic transformation in vitro culture in vitro regeneration picloram plant growth regulators somaclonal variation
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: CRS-44-1839
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||