Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: AEM-66-4372

[Submit comment/correction]

Reference	AEM-66-4372
Title	Bacterial origin and community composition in the barley phytosphere as a function of habitat and presowing conditions
Journal	Applied and Environmental Microbiology
Year	2000
Volume	66
Pages	4372-4377
Author	Normander B Prosser JI
Abstract	Summary: An understanding of the factors influencing colonization of the rhizosphere is essential for improved establishment of biocontrol agents. The aim of this study was to determine the origin and composition of bacterial communities in the developing barley (Hordeum vulgare) phytosphere, using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes amplified from extracted DNA. Discrete community compositions were identified in the endorhizosphere, rhizoplane, and rhizosphere soil of plants grown in an agricultural soil for up to 36 days. Cluster analysis revealed that DGGE profiles of the rhizoplane more closely resembled those in the soil than the profiles found in the root tissue or on the seed, suggesting that rhizoplane bacteria primarily originated from the surrounding soil. No change in bacterial community composition was observed in relation to plant age. Pregermination of the seeds for up to 6 days improved the survival of seed-associated bacteria on roots grown in soil, but only in the upper, nongrowing part of the rhizoplane. The potential occurrence of skewed PCR amplification was examined, and only minor cases of PCR bias for mixtures of two different DNA samples were observed, even when one of the samples contained plant DNA. The results demonstrate the application of culture-independent, molecular techniques in assessment of rhizosphere bacterial populations and the importance of the indigenous soil population in colonization of the rhizosphere
External Databases	Pubmed: 11010885
Keyword	[ Hide all but 1 of 36 ] 16s ribosomal rna age agricultural soil amplification bacteria bacterial community barley biocontrol biocontrol agent cluster analysis colonization community diversity dna electrophoresis gel-electrophoresis gene gradient gel-electrophoresis hordeum vulgare mixture mixtures origin pcr plant age plant dna population pseudomonads rhizobacteria rhizosphere root root tissue rrna genes seed soil survival tissue

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.