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GrainGenes Reference Report: CRS-42-105

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Reference	CRS-42-105
Title	Field evaluation of Transgenic and classical sources of Wheat streak mosaic virus resistance
Journal	Crop Science
Year	2002
Volume	42
Pages	105-110
Author	Sharp GL [ Show all 8 ]
Abstract	Summary: The development of wheat (Triticum aestivum L.) cultivars that are resistant to Wheat streak mosaic virus (WSMV), yet competitive in yield under nondiseased conditions, is an objective for breeding programs in the Great Plains. This field study was conducted to compare classical and transgenic sources of resistance to WSMV. Three sets of germplasm were evaluated. These included adapted cultivars with various levels of tolerance, transgenic wheat lines containing viral coat protein or replicase sequences from WSMV that showed resistance in greenhouse trials, and germplasm with resistance to WSMV due to a translocated segment of chromosome 4Ai-2 from Thinopyrum intermedium (Host) Barkworth and Dewey containing Wsm1. A replicated field trial was conducted at Bozeman, MT, over a two-year period to evaluate the effectiveness of these different sources of resistance to mechanical inoculation of WSMV. Adapted cultivars differed in their ability to tolerate WSMV with mean reductions in yield over the two years ranging from 41 to 74%. Incorporation of the replicase or coat protein gene from WSMV did not provide field resistance to viral infection and in general, transgenic lines yielded less than their parent cultivar, 'Hi-Line'. Wheat-Thinopyrum lines positive for a DNA marker linked to the Wsm1 gene had significantly reduced yield losses ranging from 5 to 39% compared with yield losses of 57 to 88% in near isogenic lines not having the Wsm1 gene. Yield of lines with Wsm1 in the absence of disease ranged from 11 to 28% less than yield of lines without Wsm1. Our results suggest Wsm1 provides the best source of WSMV resistance but a yield penalty may exist because of the presence of the translocation
External Databases	Pubmed: 11756260
Keyword	[ Hide all but 1 of 37 ] agropyron intermedium barley breeding breeding programs chromosome cultivar disease dna marker field resistance gene germplasm host infection inoculation intermedium isogenic lines loss marker mosaic-virus near-isogenic line protein reduction registration resistance resistant sources of resistance thinopyrum tolerance transgenic transgenic wheat translocation trials triticum triticum aestivum virus yield yield loss

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