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GrainGenes Reference Report: CRS-40-1126

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Reference	CRS-40-1126
Title	Inheritance of restriction amplification fragment length polymorphisms in perennial ryegrass
Journal	Crop Science
Year	2000
Volume	40
Pages	1126-1129
Author	Sweeney P Danneberger K
Abstract	Summary: Restriction amplification fragment length polymorphic (RAFLP) markers show promise as genetic markers in diploid outcrossing turf-grass species such as perennial ryegrass (Lolium perenne L.). The RAFLP markers should combine many advantages of restriction fragment length polymorphisms (RFLP) and random amplified polymorphic DNA (RAPD) markers and should exhibit codominance. After screening 88 primers that produced distinct fragments with five restriction enzymes, 64 restriction loci were identified. Sixteen of these appeared to be polymorphic for the restriction locus and were regarded as potential RAFLP markers. The progeny of crosses of heterozygous perennial ryegrass plants were evaluated to determine whether the proposed RAFLP markers identified in the parental plants segregated according to Mendelian genetics as predicted for codominant markers. Although 16 tentative RAFLP markers were identified, only one segregated as expected for a single locus with two alleles. Our results suggest that useful RAFLP markers will be difficult to find for outcrossing turfgrass species
Keyword	[ Hide all but 1 of 33 ] aflp alleles allozyme amplification buchloe dactyloides buffalograss codominant codominant marker creeping bentgrass cultivars dna electrophoresis enzyme fragment length polymorphism genetic markers inheritance loci locus lolium lolium perenne marker outcrossing perenne perennial ryegrass polymorphism primer progeny random amplified polymorphic dna rapd rapd markers rflp ryegrass screening turfgrass

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