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GrainGenes Reference Report: CRS-40-778

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Reference	CRS-40-778
Title	Identification of RFLP markers linked to the barley aluminum tolerance gene Alp
Journal	Crop Science
Year	2000
Volume	40
Pages	778-782
Author	Tang Y Sorrells ME Kochian LV Garvin DF
Abstract	Summary: Aluminum (Al) toxicity limits crop productivity in acidic soils. As with many crops, natural genetic variation for Al tolerance has been found in barley (Hordeum vulgare L.). Al tolerance can be evaluated by replicated field trials, by soil bioassays in pots, or by solution culture methods. To date, alternative marker-assisted methods of Al tolerance selection in barley have not been developed. The goal of this study was to identify molecular markers for Alp, a gene in the barley cv Dayton that confers a high level of Al tolerance. An F2 mapping population was generated from a cross between Dayton and the more aluminum-sensitive cv Harlan Hybrid. Al tolerance in this population was scored by hematoxylin staining, and was confirmed to segregate in a monogenic fashion. Restriction fragment length polymorphism (RFLP) mapping of Alp was undertaken, and it was localized to the long arm of chromosome 4H, 2.1 cM proximal to the marker Xbcd1117 and 2.1 cM distal to the markers Xwg464 and Xcdo1395. These markers can be used for marker-assisted selection of Al tolerance in barley without the need for field trials, soil bioassays, or solution culture analysis. Further, the linkage between Xcdo1395 and Alp is interesting because this marker is also linked to the wheat (Triticum aestivum L.) Al tolerance gene Alt(BH) located on the long arm of chromosome 4D. This result is suggestive of the possibility that Al tolerance in barley and wheat may be due to the action of orthologous loci
Keyword	[ Hide all but 1 of 38 ] acid-soil tolerance al aluminum aluminum tolerance barley bioassay chromosome chromosome location culture field fragment length polymorphism gene genetic variation hordeum vulgare hybrid inheritance isozyme limits linkage linkage map loci long arm mapping marker marker assisted selection molecular marker polymorphism population productivity rflp rflp marker scald-resistance selection soil tolerance toxicity trials triticum aestivum

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