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GrainGenes Reference Report: CRS-40-239

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Reference	CRS-40-239
Title	Improvement of bacterial blight resistance 'Minghui 63', an elite restorer line of hybrid rice, by molecular marker-assisted selection
Journal	Crop Science
Year	2000
Volume	40
Pages	239-244
Author	Chen S Lin XH Xu CG Zhang Q
Abstract	Summary: 'Minghui 63' is a restorer line widely used in hybrid rice production in China. However, this line has become increasingly susceptible to bacterial blight (BB), resulting in a rapid decline of its use in rice production. The objective of this study was to improve the BB resistance of Minghui 63 by introgressing Xa21, a broad-spectrum BB resistance gene, into Minghui 63 by molecular marker-assisted selection (MAS). A polymerase chain reaction (PCR)-based MAS system was established consisting of a marker that is a part of Xa21, a marker located at 0.8 centimorgans (cM) from the Xa21 locus on one side, and a marker at 3.0 cM from the gene on the other side. A total of 128 restriction fragment length polymorphism (RFLP) markers, evenly distributed on the 12 chromosomes were used to recover the genetic background of Minghui 63. The resulting improved version of Minghui 63, or 'Minghui 63(Xa21)', was exactly the same as the original except for a fragment of less than 3.8 cM in length surrounding the Xa21 locus. Both Minghui 63(Xa21) and its hybrid with 'Zhenshan 97A' referred to as 'Shanyou 63(Xa21)' showed the same spectrum of BB resistance as the donor parent. Field examination of a number of agronomic traits showed that Minghui 63(Xa21) and Shanyou 63(Xa21) were identical to Minghui 63 and Shanyou 63, when there was no disease stress. Under heavily diseased conditions, Minghui 63(Xa21) showed significantly higher grain weight and spikelet fertility than Minghui 63, and Shanyou 63(Xa21) was significantly higher than Shanyou 63 in grains per panicle, grain weight, and yield
Keyword	[ Hide all but 1 of 48 ] agronomic traits bacterial blight blight china chromosome maps crop yield disease disease resistance fecundity fertility field field experimentation fragment length polymorphism gene genetic background genetic markers genetic resistance grain weight grains hybrid hybrid rice improvement its loci locus marker marker assisted selection oryza oryza sativa panicles polymerase chain reaction polymorphism resistance resistance gene rflp rice seed weight selection selection criteria spectrum spikelets stress susceptibility tillers weight xa21 xanthomonas oryzae pv yield

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