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GrainGenes Reference Report: CRC-32-395

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Reference	CRC-32-395
Title	General experimental aspects for capillary electrophoretic analysis of gliadins in Triticum species
Journal	Cereal Research Communications
Year	2004
Volume	32
Pages	395-402
Author	Piergiovanni AR
Abstract	Capillary electrophoresis (CE) of gliadins from Triticum species in acidic or isoelectric buffers is currently used. The performance of 4 buffers are compared. The isoelectric buffers produced the highest resolving power and the shortest time of analysis. The slight difference in performance of the iminodiacetic acid (IDA) and aspartic acid (Asp) buffer systems should be taken into consideration before starting CE analyses. IDA buffer should be preferred when screening large germplasm collections or when a detailed characterisation of omega-gliadins is required. Asp buffer proved to be most satisfactory for cultivar discrimination or varietal identification
Keyword	[ Hide all but 1 of 9 ] analytical methods aspartic acid buffers chemical composition electrophoresis germplasm gliadin plant composition screening

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