GrainGenes Reference Report: CRC-32-301
Reference
CRC-32-301
Title
Polyglutamine-encoding microsatellite contributes to LMW GS diversity in Triticum monococcum
Journal
Cereal Research Communications
Year
2004
Volume
32
Pages
301-308
Author
Tanurdzic M Obreht D Vapa L Davidovic M
Abstract
Glu-A SUP m 3 variability of 14 accessions of diploid wheat species T. monococcum was analysed by means of SDS-PAGE and PCR. Seven tentative alleles were scored based on low molecular weight glutenin subunit (LMW GS) SDS-PAGE patterns. PCR-amplified DNA sequences corresponding to C-terminus of Glu-3 genes showed size variation that was in accordance with the B-LMW-GS variation as seen on SDS-PAGE for most of the accessions. The sequences of the amplified fragments showed that the amplicon size variation was caused solely by the expansion of the compound (CAG) SUB n (CAA) SUB n microsatellite within this region. In this paper, we have provided evidence for the first time of a correlation between the polyglutamine microsatellite and the B-LMW GS electrophoretic phenotypes
Keyword
[ Hide all but 1 of 13 ] allele diploidy diversity dna dna sequencing genetic variation glutenin microsatellite molecular weight nucleotide sequence phenotype repetitive dna sds-page
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: CRC-32-301
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||