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GrainGenes Reference Report: CCM-79-301

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Reference	CCM-79-301
Title	Synergistic and additive effects of three high molecular weight glutenin subunit loci. II. Effects on wheat dough functionality and end-use quality
Journal	Cereal Chemistry
Year	2002
Volume	79
Pages	301-307
Author	Beasley HL [ Show all 7 ]
Abstract	Summary: Understanding the relationship between basic and applied rheological parameters and the contribution of wheat flour protein content and composition in defining these parameters requires information on the roles of individual flour protein components. The high molecular weight glutenin subunit (HMW-GS) proteins are major contributors to dough strength and stability. This study focused on eight homozygous wheat lines derived from the bread wheat cvs. Olympic and Gabo with systematic deletions at each of three HMW-GS encoding gene loci, Glu-A1, Glu-B1, and Glu-D1. Flour protein levels were adjusted to a constant 9% by adding starch. Functionality of the flours was characterized by small-scale methods (2-g mixograph, microextension tester). End-use quality was evaluated by 2-g microbaking and 10-g noodle-making procedures. In this sample set, the Glu-D1 HMW-GS (5+10) made a significantly larger contribution to dough properties than HMW-GS coded by Glu-B1 (17+18), while subunit 1 coded by Glu-A1 made the smallest contribution to functionality. These differences remained after removing variations in glutenin-to-gliadin ratio. Correlations showed that both basic rheological characteristics and protein size distributions of these flours were good predictors of several applied rheological and end-use quality tests
Keyword	[ Hide all but 1 of 49 ] additive effects biochemical basis bread bread wheat chromatography component composition correlation deletion distributions dough dough properties dough property end-use quality flour flour properties flour protein flour protein content functionality gene glu-a1 glu-d1 glutenin glutenin subunit high molecular weight high molecular weight glutenin high molecular weight glutenin subunit hmw-gs homozygous information loci method mixograph molecular weight noodles parameters polymeric protein properties protein protein content quality roles size distribution stability starch subunit tester weight glutenin subunit wheat flour

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