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GrainGenes Reference Report: CCM-79-294

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Reference	CCM-79-294
Title	Synergistic and additive effects of three high molecular weight glutenin subunit loci. I. Effects on wheat dough rheology
Journal	Cereal Chemistry
Year	2002
Volume	79
Pages	294-300
Author	Uthayakumaran S [ Show all 7 ]
Abstract	Summary: The high molecular weight glutenin subunits (HMW-GS) play an important role in governing the functional properties of wheat dough. To understand the role of HMW-GS in defining the basic and applied rheological parameters and end-use quality of wheat dough, it is essential to conduct a systematic study where the effect of different HMW-GS are determined. This study focuses on the effect of HMW-GS on basic rheological properties. Eight wheat lines derived from cvs. Olympic and Gabo were used in this study. One line contained HMW-GS coded by all three loci, three lines were each null at one of the loci, three lines were null at two of the loci and one line null at all three loci. The flour protein level of all samples was adjusted to a constant 9% by adding starch. In another set of experiments, in addition to the flour protein content being held at 9%, the glutenin-to-gliadin ratio was maintained at 0.62 by adding gliadin. Rheological properties such as elongational, dynamic, and shear viscometric properties were determined. The presence of Glu-D1 subunits (5+10) made a significantly larger contribution to dough properties than those encoded by Glu-B1 (17+18), while subunit 1, encoded by Glu-A1, made the least contribution to functionality. Results also confirmed that HMW-GS contributed to strength and stability of dough
Keyword	[ Hide all but 1 of 41 ] addition additive effects biochemical basis bread wheat dough dough property dough rheology end-use quality flour flour properties flour protein flour protein content functional properties functionality gliadin glu-a1 glu-d1 glutenin glutenin subunit high molecular weight high molecular weight glutenin high molecular weight glutenin subunit hmw-gs loci parameters polymeric protein properties protein protein content quality rheological properties rheology shear stability starch starches storage proteins stress-relaxation subunit viscoelastic properties weight glutenin subunit

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