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GrainGenes Reference Report: CCM-78-737

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Reference	CCM-78-737
Title	Separation and quantification of HMW glutenin subunits by capillary electrophoresis
Journal	Cereal Chemistry
Year	2001
Volume	78
Pages	737-742
Author	Zhu J Khan K
Abstract	Summary: The use of capillary electrophoresis in SDS (SDS-CE) for separation and quantification of HMW glutenin subunits (HMW-GS) was investigated. HMW-GS were precipitated with 40% acetone from 50% 1-propanol extract of flour under reducing conditions after removal of monomeric proteins with 50% 1-propanol. Poly (ethylene oxide) was used in the running buffer (3% w/v) for SDS-CE. The results indicated that HMW-GS could be well separated by SDS-CE, including subunits 7+8, 7+9, 2+12, 5+10, and 17+18. However, HMW-GS showed delayed migration times compared with molecular weight protein standards. Some HMW-GS were reversed in their mobilities in SDS-CE compared with their mobility and molecular weights by SDS-PAGE. Therefore, the SDS-CE was unsuitable for MW determination of HMW-GS. A linear response was obtained from SDS-CE of a plot of the concentration of HMW-GS of the 40% acetone precipitate versus corrected areas for absorbance at 214 nm. Quantification of HMW-GS for the two biotypes (subunits 5+10 vs. 2+12) of an Australian wheat cultivar Warigal confirmed the differences between the two biotypes in their quantity of HMW-GS. Therefore, the technique could be used to quantify HMW-GS in conjunction with SDS-PAGE. Total number of records in report: 118
Keyword	[ Hide all but 1 of 32 ] allelic variation australian wheat biotype biotypes breadmaking quality capillary electrophoresis cereal storage proteins cultivar cultivar differentiation electrophoresis ethylene flour glutenin glutenin subunit hmw glutenin hmw glutenin subunit migration mobility molecular weight molecular weight subunits protein quantification quantities removal sds sds page separation subunit varietal identification wheat cultivar wheat proteins zone electrophoresis

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