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GrainGenes Reference Report: CCM-76-650

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Reference
CCM-76-650
Title
Soft wheat quality as related to protein composition
Journal
Cereal Chemistry
Year
1999
Volume
76
Pages
650-655
Author
Huebner FR
Bietz JA
Nelsen T
Bains GS
Finney PL
Abstract
Summary: Soft red and white winter wheats from the eastern United States, used primarily to produce cookies, cakes, and biscuits, have quality requirements very different from those of bread wheats. In general, soft wheats have been bred to have low protein content, and conventional wisdom has been that protein composition of soft wheat is relatively unimportant. To test this hypothesis, relationships between soft wheat protein composition and end-use functional quality characteristics were examined. Quantitative protein compositions of eight cultivars of soft wheats grown in a wide area of the eastern United States during seven years (53 samples total) were analyzed by size-exclusion HPLC. Results were statistically correlated with numerous chemical and physical characteristics and quality factors of these wheats, their flours, and of cookies baked from the flours. For the entire sample set, wheats containing high molecular weight glutenin subunits 2+12 showed significantly different properties and cookie characteristics from those with subunits 5+10, but amounts of most individual fractions correlated poorly with quality descriptors. For individual soft wheat cultivar, however, amounts of many individual gliadin and glutenin subfractions correlated significantly with quality descriptors such as SDS sedimentation, mixograph absorption, peak mixing time, mixograph number, cookie diameter, and top grain. Protein contents as a function of genotype and environment also differed greatly among cultivars, as did ratios of gliadin to glutenin. These results clearly revealed that suitability of soft wheat cultivar for specific products can be rapidly determined by quantitative and qualitative analyses of protein composition
Keyword
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analytical methods
area
baking quality
bread
bread wheat
cultivar
flour
food analysis
genotype
gliadin
glutenin
mixing
molecular weight
protein composition
protein content
quality
quality characteristics
rapid methods
ratios
weight
wheat flour
wheat protein
winter
winter wheat

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