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GrainGenes Reference Report: PGM-9:e700003

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Reference	PGM-9:e700003
Title	Characterization of a new Lr52 allele for leaf rust resistance in the Iranian wheat landrace PI 622111
Journal	Plant Genome
Year	2025
Volume	18
Author	Xu X [ Show all 8 ]
Abstract	Leaf rust, caused by Puccinia triticina (Pt), poses a constant threat to global wheat production, and novel leaf rust resistance genes are needed to combat the disease. A previous genome-wide association study (GWAS) identified a single nucleotide polymorphism (SNP) marker associated with leaf rust resistance in the terminal region of chromosome arm 5BS in the Iranian landrace PI 622111. An F2 population and 175 F2:3 families from cross PI 62211 x Yuanyu 3 were evaluated for response to Pt isolate Pt52-2 (MMPSD). Genotyping-by-sequencing analysis and genotyping of a subset of the F2 plants identified 32 SNPs closely associated with leaf rust resistance in the target region. Some of these SNPs were converted into kompetitive allele-specific polymorphic (KASP) markers and used to genotype the F2 population together with a set of simple sequence repeat (SSR) markers also located in the target genomic region. Linkage analysis delimited the leaf rust resistance gene in PI 622111, designated Lr622111, to a 0.4 Mb interval flanked by Xstars700 (7.22 Mb) and Xstars678 (7.62 Mb) in IWGSC RefSeq v.2.1. An allelism test involving 811 F2 plants indicated that Lr622111 was allelic to Lr52. Since PI 622111 reacted differently from the Lr52 donor to Pt races in the GWAS, Lr622111 is considered a new Lr52 allele conferring a wide spectrum of resistance to current US Pt races. KASP marker Xstars-KASP239, which is 0.9 cM distal to Lr622111, can be widely used to tag Lr622111 in breeding populations.
External Databases	http://doi.ort/10.1002/tpg2.70003
Allele	Lr622111 (Triticum)
Gene	Lr52 (Triticum)
Germplasm	PI 622111
Locus	Xstars700 Xstars678 Xstars-KASP239
Probe	[ Hide all but 1 of 18 ] Xstars661 Xstars665 Xstars668 Xstars669 Xstars678 Xstars680 Xstars682 Xstars689 Xstars691 Xstars692 Xstars699 Xstars700 Xstars-KASP710 Xstars-KASP731 Xstars-KASP239 Xstars-KASP728 Xstars-KASP67 Xstars-KASP716

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