GrainGenes Reference Report: TAG-2022-Athiyannan
Reference
TAG-2022-Athiyannan
Title
Haplotype variants of the stripe rust resistance gene Yr28 in Aegilops tauschii
Journal
Theoretical and Applied Genetics
Year
2022
Remark
Published online Sept 2022
Author
[ Hide all but 1 of 17 ] Athiyannan N Zhang P McIntosh R Chakraborty S Hewitt T Bhatt D Forrest K Upadhyaya N Steuernagel B Arora S Huerta J Hayden M Wulff BBH Ayliffe M Hickey LT Lagudah E Periyannan S
Abstract
New sources of host resistance are required to counter the continued emergence of new pathotypes of the wheat stripe rust pathogen Puccinia striiformis Westend. f. sp. tritici Erikss. (Pst). Here, we show that CPI110672, an Aegilops tauschii accession from Turkmenistan, carries a single Pst resistance gene, YrAet672, that is effective against multiple Pst pathotypes, including the four predominant Pst lineages present in Australia. The YRAet672 locus was fine mapped to the short arm of chromosome 4D, and a nucleotide-binding and leucine-rich repeat gene was identified at the locus. A transgene encoding the YrAet672 genomic sequence, but lacking a copy of a duplicated sequence present in the 3' UTR, was trans- formed into wheat cultivar Fielder and Avocet S. This transgene conferred a weak resistance response, suggesting that the duplicated 3' UTR region was essential for function. Subsequent analyses demonstrated that YrAet672 is the same as two other Pst resistance genes described in Ae. tauschii, namely YrAS2388 and Yr28. They were identified as haplotypes encoding identical protein sequences but are polymorphic in non-translated regions of the gene. Suppression of resistance conferred by YrAet672 and Yr28 in synthetic hexaploid wheat lines (AABBDD) involving Langdon (AABB) as the tetraploid parent was associated with a reduction in transcript accumulation.
External Databases
https://doi.org/10.1007/s00122-022-04221-w
Gene
Yr28 (Triticum)
Germplasm
Aegilops tauschii CPI110672
Locus
Yr28
Probe
KASPYr28
Sequence
KASPYr28
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-2022-Athiyannan
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||