GrainGenes Reference Report: PBR-132-563
Reference
PBR-132-563
Title
Application of markerâ€assisted selection and genomeâ€wide association scanning to the development of winter food barley germplasm resources
Journal
Plant Breeding
Year
2013
Volume
132
Pages
563-570
Author
[ Hide all but 1 of 10 ] Chutimanitsakun Y Cuesta-Marcos A Chao S Corey A Filichkin T Fisk S Kolding M Meints B Ong Y-L Rey JI
Abstract
Barley (Hordeum vulgare) is an important component of heart-healthy whole grain diets because it contains β-glucan. All current US barley varieties with high β-glucan are spring habit and have waxy starch. Winter varieties have agronomic advantages but require low-temperature tolerance (LTT). Vernalization sensitivity (VS) is associated with higher levels of LTT. To rapidly develop fall-sown varieties with LTT and higher grain beta-glucan, we therefore used marker-assisted selection (MAS) at the WX and VRN-H2 loci. The MAS-derived lines, together with unrelated non-waxy germplasm developed via phenotypic selection (PS), were used for a genome-wide association scan (GWAS). The panel was phenotyped for grain beta-glucan, LTT and VS. It was genotyped with 3072 single-nucleotide polymorphisms (SNPs) and allele-specific primers. Marker-assisted selection fixed target alleles at both loci but only one of the target phenotypes (higher beta-glucan percentage) was achieved. Variation for VS and LTT is attributable to (i) incomplete information about VRN-H1 at the outset of the project and (ii) unexpected allelic variation at VRN-H3 with a large effect on VS and LTT.
External Databases
https://doi.org/10.1111/pbr.12086
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PBR-132-563
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||