GrainGenes Reference Report: CAM-7-465
Reference
CAM-7-465
Title
The genetic structure of tetraploid Avena : a comparison of isozyme and RAPD markers
Journal
Cellular and Molecular Biology Letters
Year
2002
Volume
7
Pages
465-469
Author
Mohammed B Guma R Perez de la Vega M Garcia P
Abstract
Isozymes were the first widely used molecular markers in plant population analysis. They yielded valuable information on the amount and the structure of genetic variability. DNA technology has provided new types of markers based on DNA sequence, which make it possible to study polymorphisms in a much greater proportion of the genome. This is the reason why the use of isozymes is less popular nowadays. This effect would be justified if all markers provided the same type of information on polymorphism and genetic relationships among populations; otherwise, it would be necessary to use different markers to obtain the complete picture of the genetic structure of populations and species. In this study, we compared data of isozyme and RAPD markers in the populations of two tetraploid species of wild oats: Avena barbata populations collected in Argentina, and Avena murphyi populations collected in Spain and Morocco. The samples were evaluated for 9 isozymatic systems and 10 primers. The structure of genetic variability was studied using Nei's method, and the relationships between populations were estimated using Hedrick and Jaccard's similarities for isozymes and RAPDs, respectively. As expected, RAPDs were more polymorphic than isozymes, but the information obtained from both markers was weakly correlated. The various reasons for this observation are discussed, but our conclusion is that in order to study the structure of genetic variability, several types of markers should be used
External Databases
Pubmed: 12378251
Keyword
[ Hide all but 1 of 11 ] dna enzyme enzyme polymorphism genetic marker genetic polymorphism genetic variation genome isoenzyme oat random amplified polymorphicdna structure
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: CAM-7-465
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||