GrainGenes Reference Report: TAG-133-1109
Reference
TAG-133-1109
Title
Chromosomal location of the crown rust resistance gene Pc98 in cultivated oat (Avena sativa L.)
Journal
Theoretical and Applied Genetics
Year
2020
Volume
133
Pages
1109-1122
Author
Zhao J
[ Show all 9 ]
Abstract
Crown rust is among the most damaging diseases of oat and is caused by Puccinia coronata var. avenae f. sp. avenae (Urban and Marková) (Pca). Host resistance is the preferred method to prevent crown rust epidemics. Pc98 is a race-specific, seedling crown rust resistance gene obtained from the wild oat Avena sterilis accession CAV 1979 that is effective at all growth stages of oat. Virulence to Pc98 has been very low in the Pca populations that have been tested. The objectives of this study were to develop SNP markers linked to Pc98 for use in marker-assisted selection and to locate Pc98 on the oat consensus map. The Pc98 gene was mapped using F2:3 populations developed from the crosses Pc98/Bingo and Pc98/Kasztan, where Pc98 is a single-gene line carrying Pc98. Both populations were evaluated in seedling inoculation experiments. Pc98 was mapped relative to Kompetitive Allele-Specific PCR SNP markers in both populations, placing Pc98 on the Mrg20 linkage group of the consensus map. Pc98 was bracketed by two SNP markers GMI_ES22_c3052_382_kom399 and GMI_ES14_lrc18344_662_kom398 in the Pc98/Bingo mapping population with genetic distances of 0.9 cM and 0.3 cM, respectively. Pc98 co-segregated with four SNP markers in the Pc98/Kasztan population, and the closest flanking markers were GMI_DS_LB_6017_kom367 and avgbs2_153634.1.59_kom410 with genetic distances of 0.7 cM and 0.3 cM, respectively. Two SNP loci defined a haplotype that accurately predicted Pc98 status in a diverse group of oat germplasm, which will be valuable for marker-assisted selection of Pc98 in breeding of new oat cultivars.
External Databases
https://doi.org/10.1007/s00122-020-03535-x
Gene
Pc98 (Avena)
Germplasm
Pc98 (oat) PI 411502 CAV 1979 Bingo (oat) Kasztan (oat)
Locus
GMI_ES02_lrc18492_57
[ Show all 21 ]
Map Data
Oat-2020-Pc98
Probe
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-133-1109
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||