GrainGenes Reference Report: TAG-131-721
Reference
TAG-131-721
Title
Identification, introgression, and molecular marker genetic analysis and selection of a highly effective novel oat crown rust resistance from diploid oat, Avena strigosa
Journal
Theoretical and Applied Genetics
Year
2018
Volume
131
Pages
721-733
Author
Rines HW
[ Show all 7 ]
Abstract
A new highly effective resistance to oat crown rust (Puccinia coronata f. sp. avenae) was identified in the diploid oat Avena strigosa PI 258731 and introgressed into hexaploid cultivated oat. Young plants with this resistance show moderate susceptibility, whereas older plant tissues and adult plants are resistant with no virulent isolates encountered in over 8 years of testing. Resistance was incorporated into hexaploid oat by embryo rescue, colchicine chromosome doubling followed by backcrosses with a hexaploid parent, and selection for stable transmission of resistance. To mitigate flag leaf and panicle chlorosis/necrosis associated with the resistance, crosses were made with derived resistant lines to breeding lines of divergent parentage followed by selection. Subsequently, two F2 sister lines, termed MNBT1020-1 and MNBT1021-1, were identified in which the chlorosis/necrosis was reduced. These two lines performed well in replicated multi-location state trials in 2015 and 2016 out-yielding all cultivar entries. Segregating F2:3 plants resulting from crosses of MNBT lines to susceptible parents were genotyped with the oat 6K SNP array, and SNP loci with close linkage to the resistance were identified. KASP assays generated from linked SNPs showed accurate discrimination of the resistance in derivatives of the resistant MNBT lines crossed to susceptible breeding lines. A TaqMan marker was developed and correctly identified homozygous resistance in over 95% of 379 F4 plants when rust was scored in F4:5 plants in the field. Thus, a novel highly effective resistance and associated molecular markers are available for use in breeding, genetic analysis, and functional studies.
External Databases
https://doi.org/10.1007/s00122-017-3031-0
Gene
Pc_PI258731 (Avena)
Germplasm
BetaGene (oat) PI 258731
Locus
[ Hide all but 1 of 8 ] Pc_PI258731 KASP_GMI_ES15_c2369_181 KASP_GMI_ES15_c7632_384 KASP_GMI_ES02_c37788_255 KASP_GMI_ES03_c6094_414 KASP_GMI_ES13_c626_111 KASP_GMI_ES03_c2957_770 KASP_GMI_ES03_c6181_441
Map Data
Oat-2018-Pc_PI258731
Probe
KASP_GMI_ES15_c2369_181
[ Show all 7 ]
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-131-721
|
| ||||||||||
|
| ||||||||||
|
| ||||||||||
|
| ||||||||||
|
| ||||||||||
|
| ||||||||||
|
| ||||||||||
|
| ||||||||||
|
| ||||||||||
|
| ||||||||||
|
| ||||||||||
|
| ||||||||||
|
| ||||||||||
|
|
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||