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GrainGenes Reference Report: TAG-133-857

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Reference	TAG-133-857
Title	QTL mapping for grain yield-related traits in bread wheat via SNP-based selective genotyping
Journal	Theoretical and Applied Genetics
Year	2020
Volume	133
Pages	857-872
Author	Yang L [ Show all 10 ]
Abstract	Identification of major stable quantitative trait loci (QTL) for grain yield-related traits is important for yield potential improvement in wheat breeding. In the present study, 266 recombinant inbred lines (RILs) derived from a cross between Zhongmai 871 (ZM871) and its sister line Zhongmai 895 (ZM895) were evaluated for thousand grain weight (TGW), grain length (GL), grain width (GW), and grain number per spike (GNS) in 10 environments and for grain filling rate in six environments. Sixty RILs, with 30 higher and 30 lower TGW, respectively, were genotyped using the wheat 660 K SNP array for preliminary QTL mapping. Four genetic regions on chromosomes 1AL, 2BS, 3AL, and 5B were identified to have a significant effect on TGW-related traits. A set of Kompetitive Allele Specific PCR markers were converted from the SNP markers on the above target chromosomes and used to genotype all 266 RILs. The mapping results confirmed the QTL named Qgw.caas-1AL, Qgl.caas-3AL, Qtgw.caas-5B, and Qgl.caas-5BS on the targeted chromosomes, explaining 5.0 - 20.6%, 5.7 - 15.7%, 5.5 - 17.3%, and 12.5 - 20.5% of the phenotypic variation for GW, GL, TGW, and GL, respectively. A novel major QTL for GNS on chromosome 5BS, explaining 5.2 - 15.2% of the phenotypic variation, was identified across eight environments. These QTL were further validated using BC1F4 populations derived from backcrosses ZM871/ZM895//ZM871 (121 lines) and ZM871/ZM895//ZM895 (175 lines) and 186 advanced breeding lines. Collectively, selective genotyping is a simple, economic, and effective approach for rapid QTL mapping and can be generally applied to genetic mapping studies for important agronomic traits.
External Databases	https://doi.org/10.1007/s00122-019-03511-0
Germplasm	Zhongmai 871 Zhongmai 895
Locus	Kasp_1A10 [ Show all 26 ]
Map Data	Wheat-2019-KASP_YldQTL
Probe	[ Hide all but 1 of 26 ] Kasp_1A10 Kasp_1A58 Kasp_1A64 Kasp_1A90 Kasp_2B13 Kasp_2B16 Kasp_2B51 Kasp_2B55 Kasp_2B58 Kasp_2B72 Kasp_3A10 Kasp_3A11 Kasp_3A14 Kasp_3A5 Kasp_3A7 Kasp_3A8 Kasp_3A9 Kasp_5B11 Kasp_5B12 Kasp_5B2 Kasp_5B3 Kasp_5B4 Kasp_5B5 Kasp_5B6 Kasp_5B8 Kasp_5B9
QTL	Qtgw.caas-1AL [ Show all 15 ]

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