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GrainGenes Reference Report: GGG-9-505

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Reference	GGG-9-505
Title	Mapping Oat Crown Rust Resistance Gene Pc45 Confirms Association with PcKM
Journal	G3: Genes, Genomes, Genetics
Year	2019
Volume	9
Pages	505-511
Author	Kebede AZ [ Show all 9 ]
Abstract	Molecular mapping of crown rust resistance genes is important to effectively utilize these genes and improve breeding efficiency through marker-assisted selection. Pc45 is a major race-specific crown rust resistance gene initially identified in the wild hexaploid oat Avena sterilis in the early 1970s. This gene was transferred to cultivated oat (Avena sativa) and has been used as a differential for identification of crown rust races since 1974. Previous research identified an association between virulence to Pc45 and PcKM, a crown rust resistance gene in the varieties 'Kame' and 'Morton'. This study was undertaken to reveal the relationship between Pc45 and PcKM. Pc45 was studied in the crosses 'AC Morgan'/Pc45 and 'Kasztan'/Pc45, where Pc45 is the differential line carrying Pc45. F2 progenies and F2:3 families of both populations were inoculated with the crown rust isolate CR258 (race NTGG) and single gene segregation ratios were observed. SNP markers for PcKM were tested on these populations and linkage maps were generated. In addition, 17 newly developed SNP markers identified from genotyping-by-sequencing (GBS) data were mapped in these two populations, plus another three populations segregating for Pc45 or PcKM. Pc45 and PcKM mapped to the same location of Mrg08 (chromosome 12D) of the oat chromosome-anchored consensus map. These results strongly suggest that Pc45 and PcKM are the same resistance gene, but allelism (i.e., functionally different alleles of the same gene) or tight linkage (i.e., two tightly linked genes) cannot be ruled out based on the present data.
External Databases	https://doi.org/10.1534/g3.118.200757
Locus	Pc45
Map Data	Oat-2019-CrownRust
Probe	[ Hide all but 1 of 34 ] avgbs_105241.1_KASP avgbs_106741.1_KASP avgbs_114987.1_KASP avgbs_119797.1_KASP avgbs_12128.1_KASP avgbs_213780_KASP avgbs_61564_KASP avgbs_85697.1_KASP avgbs_89273.1_KASP avgbs_99510.1_KASP avgbs_cluster_10762.1_KASP avgbs_cluster_12723.1_KASP avgbs_cluster_36254.1_KASP avgbs_cluster_5231.1_KASP avgbs2_134349.1_KASP avgbs2_172881.1_KASP avgbs2_37871.1_KASP avgbs2_51444.1_KASP avgbs2_55761.1_KASP GMI_DS_LB_1354_KASP GMI_DS_oPt-17084_293_KASP GMI_ES_CC11253_379_KASP GMI_ES01_c26749_466_KASP GMI_ES02_c13596_178_KASP GMI_ES02_c16987_268_KASP GMI_ES02_c3359_447_KASP GMI_ES02_c8737_267_KASP GMI_ES03_c4125_413_KASP I05-0090-KOM13c1_KASP I05-0407-KOM14c1_KASP I05-0601-KOM15c2_KASP I05-0874-KOM16c1_KASP I05-1033-KOM17c1_KASP I05-1143-KOM18c1_KASP

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