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GrainGenes Reference Report: PBR-126-274

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Reference	PBR-126-274
Title	EST-derived microsatellites as a rich source of molecular markers for oats
Journal	Plant Breeding
Year	2007
Volume	126
Pages	274-278
Author	Becher R
Abstract	Polymerase chain reaction-based microsatellite markers are valuable tools for molecular breeding because of their co-dominant inheritance and their applicability for high-throughput analysis. Being still very limited for oats, their number has to be increased significantly to cover the entire genome. For these purposes, a set of 7031 recently published expressed sequence tags (ESTs) was screened for microsatellites with dinucleotide, trinucleotide and tetranucleotide repeat motifs. Subsequent in silico analysis resulted in the development of 216 primer pairs for Avena EST-derived microsatellite loci (AME). Using a sample set of 12 oat lines, 107 of 195 functional primers could be assayed as polymorphic. The marker variability averaged out at three alleles per locus and a polymorphic information content (PIC) value of 0.42. This variability documents their suitability for molecular oat-breeding purposes. Finally, 51 of the AME loci could be placed within the known reference map of 'Kanota' x 'Ogle' that previously contained only 12 microsatellite loci. Thus, a remarkable enhancing of the number of mapped oat microsatellite loci could be achieved.
External Databases	https://doi.org/10.1111/j.1439-0523.2007.01330.x
Locus	[ Hide all but 1 of 221 ] AME041 AME001 AME002 AME003 AME004 AME005 AME006 AME007 AME008 AME009 AME010 AME011 AME012 AME013 AME014 AME015 AME016 AME017 AME018 AME019 AME019a AME019b AME020 AME021 AME022 AME023 AME023a AME023b AME023c AME024 AME025 AME026 AME027 AME027-2 AME028 AME029 AME030 AME031 AME031-2 AME032 AME033 AME034 AME035 AME036 AME037 AME038 AME039 AME040 AME042 AME043 AME044 AME045 AME046 AME047 AME048 AME049 AME050 AME051 AME052 AME053 AME054 AME055 AME056 AME057 AME058 AME059 AME060 AME061 AME062 AME063 AME064 AME065 AME066 AME067 AME067-2 AME068 AME069 AME070 AME071 AME072 AME073 AME074 AME075 AME076 AME077 AME078 AME079 AME080 AME081 AME082 AME083 AME084 AME085 AME086 AME087 AME088 AME089 AME090 AME090-2 AME091 AME092 AME093 AME094 AME095 AME096 AME097 AME098 AME099 AME100 AME101 AME102 AME103 AME104 AME105 AME106 AME107 AME108 AME108-2 AME109 AME110 AME111 AME112 AME113 AME114 AME115 AME116 AME117 AME118 AME119 AME120 AME121 AME121-2 AME122 AME123 AME123-2 AME124 AME125 AME126 AME127 AME128 AME129 AME130 AME131 AME132 AME132-2 AME133 AME134 AME135 AME136 AME137 AME138 AME139 AME140 AME141 AME142 AME143 AME144 AME145 AME145-2 AME146 AME147 AME148 AME149 AME150 AME151 AME152 AME153 AME153-2 AME154 AME155 AME156 AME157 AME158 AME159 AME160 AME161 AME162 AME162-2 AME163 AME164 AME165 AME166 AME167 AME168 AME169 AME170 AME171 AME172 AME173 AME174 AME175 AME176 AME177 AME178 AME179 AME180 AME181 AME182 AME183 AME184 AME185 AME186 AME187 AME188 AME189 AME190 AME191 AME192 AME193 AME194 AME195 AME196 AME197 AME198 AME199 AME200 AME201 AME202 AME203 AME204 AME205
Probe	AME41 [ Show all 216 ]

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