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GrainGenes Reference Report: SCD-4:170044

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Reference	SCD-4:170044
Title	Construction of a map-based reference genome sequence for barley, Hordeum vulgare L.
Journal	Scientific Data
Year	2017
Volume	4:170044
Author	[ Hide all but 1 of 60 ] Beier S Himmelbach A Colmsee C Zhang X-Q Barrero RA Zhang Q Li L Bayer M Bolser D Taudien S Groth M Felder M Hastie A Simkova H Stankova H Vrana J Chan S Munoz-Amatriain M Ounit R Wanamaker S Schmutzer T Aliyeva-Schnorr L Grasso S Tanskanen J Sampath D Heavens D Cao S Chapman B Dai F Han Y Li H Li X Lin C McCooke JK Tan C Wang S Yin S Zhou G Poland JA Bellgard MI Houben A Dolezel J Ayling S Lonardi S Langridge P Muehlbauer GJ Kersey P Clark MD Caccamo M Schulman AH Platzer M Close TJ Hansson M Zhang G Braumann I Li C Waugh R Scholz U Stein N Mascher M
Abstract	Barley (Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. 'Morex' was constructed by the International Barley Genome Sequencing Consortium (IBSC) using hierarchical shotgun sequencing. Here, we report the experimental and computational procedures to (i) sequence and assemble more than 80,000 bacterial artificial chromosome (BAC) clones along the minimum tiling path of a genome-wide physical map, (ii) find and validate overlaps between adjacent BACs, (iii) construct 4,265 non-redundant sequence scaffolds representing clusters of overlapping BACs, and (iv) order and orient these BAC clusters along the seven barley chromosomes using positional information provided by dense genetic maps, an optical map and chromosome conformation capture sequencing (Hi-C). Integrative access to these sequence and mapping resources is provided by the barley genome explorer (BARLEX).
External Databases	https://doi.org/10.1038/sdata.2017.44
Locus	HORVU1Hr1G000010.3 [ Show all 39735 ]
Map Data	Barley, 2017, IBSC Morex v1
Probe	HORVU1Hr1G000010 [ Show all 31899 ]
Sequence	HORVU1Hr1G000010.3 [ Show all 39734 ]

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