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GrainGenes Reference Report: TAG-122-317

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Reference	TAG-122-317
Title	Mapping of the oat crown rust resistance gene Pc91.
Journal	Theoretical and Applied Genetics
Year	2011
Volume	122
Pages	317-325
Author	McCartney CA [ Show all 8 ]
Abstract	Crown rust is an important disease of oat caused by Puccinia coronata Corda f. sp. avenae Eriks. Crown rust is efficiently and effectively managed through the development of resistant oat varieties. Pc91 is a seedling crown rust resistance gene that is highly effective against the current P. coronata population in North America. The primary objective of this study was to develop DNA markers linked to Pc91 for purposes of marker-assisted selection in oat breeding programs. The Pc91 locus was mapped using a population of F7-derived recombinant inbred lines developed from the cross 'CDC Sol-Fi'/'HiFi' made at the Crop Development Centre, University of Saskatchewan. The population was evaluated for reaction to P. coronata in field nurseries in 2008 and 2009. Pc91 mapped to a linkage group consisting of 44 Diversity Array Technology (DArT) markers. DArTs were successfully converted to sequence characterized amplified region (SCAR) markers. Five robust SCARs were developed from three non-redundant DArTs that co-segregated with Pc91. SCAR markers were developed for different assay systems, such that SCARs are available for agarose gel electrophoresis, capillary electrophoresis, and Taqman single nucleotide polymorphism detection. The SCAR markers accurately postulated the Pc91 status of 23 North American oat breeding lines.
External Databases	https://doi.org/10.1007/s00122-010-1448-9 https://link.springer.com/article/10.1007/s00122-010-1448-9
Locus	[ Hide all but 1 of 49 ] oPt-17475 oPt-0120 oPt-0350 oPt-0453 oPt-0615 oPt-10068 oPt-11327 oPt-11446 oPt-11506 oPt-12034 oPt-12195 oPt-12215 oPt-12650 oPt-12861 oPt-13202 oPt-13897 oPt-14381 oPt-14477 oPt-14509 oPt-15259 oPt-15309 oPt-15597 oPt-15736 oPt-16230 oPt-1652 oPt-16558 oPt-17240 oPt-1817 oPt-18177 oPt-18233 oPt-3109 oPt-3191 oPt-3192 oPt-3722 oPt-5343 oPt-5495 oPt-5552 oPt-7996 oPt-8250 oPt-8794 oPt-8892 oPt-9234 oPt-9423 oPt-9546 oPt-0350-cdc1 oPt-0350-cdc2 oPt-0350-cdc3 oPt-9234-cdc1 oPt-9546-cdc1
Map Data	Oat-2010-SolxHi
Probe	oPt-0350-cdc1 oPt-0350-cdc2 oPt-0350-cdc3 oPt-9234-cdc1 oPt-9546-cdc1

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