GrainGenes Reference Report: BPL-48-313
Reference
BPL-48-313
Title
Regeneration capacity of calli derived from immature embryos in spring barley cultivars
Journal
Biologia Plantarum
Year
2004
Volume
48
Pages
313-316
Author
Halamkova E Vagera J Ohnoutkova L
Abstract
Callogenesis, somatic embryogenesis and regeneration capacity in twenty-three agronomically important spring barley ( Hordeum vulgare ) cultivars on induction media with 2,4-dichlorophenoxyacetic acid (2,4-D) or 3,6-dichloro- o -anisic acid (dicamba) and on modified regeneration media were studied. The frequency of zygotic embryos exhibiting callogenesis varied from 88 to 100% according to cultivar. Dicamba was more suitable for somatic embryogenesis induction and exhibited a higher frequency of regenerants than did 2,4-D. Green regenerants were obtained in all cultivars, and there were no albino plants. Except for Victor all cultivars used in the experiment showed lower regeneration capacity compared to the model Golden Promise
Keyword
[ Hide all but 1 of 15 ] 2,4-d barley callus cultivar dicamba embryogenesis herbicides in vitro culture in vitro regeneration plant growth regulators regeneration somatic embryogenesis spring tissue culture zygotes
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: BPL-48-313
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||