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GrainGenes Reference Report: BPL-48-179

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Reference	BPL-48-179
Title	Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L
Journal	Biologia Plantarum
Year	2004
Volume	48
Pages	179-184
Author	Mitic N Nikolic R Ninkovic S Miljus-Djukic J Neskovic M
Abstract	The use of two A. tumefaciens strains for transformation of T. aestivum cv. Vesna was studied. Immature embryos, isolated 15 days after pollination, were cocultivated with the super-binary LBA4404/pTOK233 and the binary AGL1/pDM805 vectors. While the transient GUS-intron expression was high (69.9 and 80.0%), the number of plants regenerated on selective media containing hygromycin or phosphinotricin did not exceed 0.4 and 0.13%, respectively. Nevertheless, the regenerated plants were fertile and produced seeds. The T SUB 0 plants, as well as the T SUB 1 seedlings, displayed the activity in the beta-glucuronidase histochemical assay and a positive signal in PCR analysis for the presence of uidA gene sequences in their genomes. The data suggest that the transformation of wheat cv. Vesna with both Agrobacterium strains is feasible
Keyword	[ Hide all but 1 of 33 ] agrobacterium agrobacterium tumefaciens agrobacterium-mediated transformation assay bar gene beta-glucuronidase bombardment fertile gene gene sequence genetic-transformation gus expression hordeum vulgare hpt gene immature embryo marker media pcr pcr analysis plants regenerated pollination regeneration selective medium sequence signal t-dna transformation transgenic plant triticum triticum aestivum triticum aestivum-l vector vectors

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