GrainGenes Reference Report: EMB-14-2715
Reference
EMB-14-2715
Title
A new type of signal peptide: central role of a twin-arginine motif in transfer signals for the delta pH-dependent thylakoidal protein translocase.
Journal
The EMBO Journal
Year
1995
Volume
14
Pages
2715-2722
Author
[ Hide all but 1 of 7 ] Chaddock A Mant A Karnauchov I Brink S Herrmann R Klosgen R Robinson C
Abstract
The delta pH-driven and Sec-related thylakoidal protein translocases recognise distinct types of thylakoid transfer signal, yet all transfer signals resemble bacterial signal peptides in structural terms Comparison of known transfer signals reveals a single concrete difference: signals for the delta pH-dependent system contain a common twin-arginine motif immediately before the hydrophobic region We show that this motif is critical for the delta pH-driven translocation process; substitution of the arg-arg by gin-gin or even arg-lys totally blocks translocation across the thylakoid membrane, and replacement by lys-arg reduces the rate of translocation by > 100-fold The targeting information in this type of signal thus differs fundamentally from that of bacterial signal peptides, where the required positive charge can be supplied by any basic amino acid Insertion of a twin-arg motif into a Sec-dependent substrate does not alter the pathway followed but reduces translocation efficiency, suggesting that the motif may also repel the Sec-type system Other information must help to specify the choice of translocation mechanism, but this information is unlikely to reside in the hydrophobic region because substitution by a hydrophobic section from an integral membrane protein does not affect the translocation pathway
Keyword
amino acid sequence
[ Show all 16 ]
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: EMB-14-2715
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
|
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||