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GrainGenes Reference Report: CPP-26-89

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Reference	CPP-26-89
Title	Development of sequence-characterized amplified region (SCAR) markers for resistance gene Pc94 to crown rust in oat
Journal	Canadian Journal of Plant Pathology
Year	2004
Volume	26
Pages	89-96
Author	Chong J Reimer E Somers D Aung T Penner GA
Abstract	Crown rust, caused by Puccinia coronata f. sp. avenae, is an important disease of oat (Avena sativa). Pyramiding effective resistance from diverse sources is one strategy to provide more durable control of crown rust. The objective of this study was to develop a DNA marker for the resistance gene Pc94 to crown rust derived from the diploid oat Avena strigosa. Amplified fragment length polymorphism generated two DNA fragments that were associated with the resistance gene Pc94 in a F2 population derived from a cross between the rust-susceptible oat 'Calibre' and a near-isogenic hexaploid oat line, S42, possessing Pc94. One of the fragments was successfully converted into two sequence-characterized amplified region (SCAR) markers linked to the Pc94 gene in the Calibre/S42 F2 population. Specificity of the SCAR markers to the Pc94 gene was confirmed in three other populations. The estimated distance between the SCAR markers and Pc94 ranged from 0.9 to 3.4 centi-Morgan. These SCAR markers developed for the Pc94 gene will be valuable tools for pyramiding this gene with other resistance genes to crown rust in marker-assisted breeding programs when crown-rust races with the appropriate virulence combinations are not available to detect the genes being introgressed.
External Databases	https://doi.org/10.1080/07060660409507118
Gene	Pc-94 (Avena)
Germplasm	S42 (oat)
Locus	Pc94 SCAR94-1 SCAR94-2
Map Data	Oat-2004-CalxS42 Oat-2004-MS68xS42
Probe	SCAR94-1 SCAR94-2
Keyword	[ Hide all but 1 of 41 ] aflp marker amplified fragment length polymorphism avena sativa avena strigosa barley breeding breeding program canada control crown rust diploid disease distance dna dna marker f-2 population f-sp avenae f2 fragment length polymorphism fragments gene hexaploid molecular marker oat oat crown rust polymorphism puccinia pyramiding race races rapd marker resistance rust scar scar markers sequence-characterized amplified region specificity strategy tool tools virulence

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