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GrainGenes Reference Report: CPP-21-308

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Reference
CPP-21-308
Title
Genetic diversity amoing Fusarium graminearum strains from Ontario and Quebec
Journal
Canadian Journal of Plant Pathology
Year
1999
Volume
21
Pages
308-314
Author
Dusabenyagasani M
Dostaler D
Hamelin RC
Abstract
Summary: The random amplified polymorphic DNA (RAPD) method was used to sample DNA markers from 72 strains of Fusarium graminearum isolated from wheat blight kernels collected from Quebec (52 strains), Ontario (18 strains), and Prince Edward Island (2 strains). Sixty-five markers generated with eight RAPD primers showed that all strains were genetically distinct. Moreover, the analysis of molecular variance (AMOVA) indicated that 90.56% of the genetic variability associated with strains used in this study was explained by within-region variation. The G(ST) values were low and showed that there was almost no genetic differentiation associated with sampling F. graminearum either from two different years in Quebec (G(ST)=0.0375) or from Ontario and Quebec (G(ST)=0.0509). These results show that F. graminearum strains from Quebec and Ontario might be part of a single population pool
Keyword
[ Hide all but 1 of 29 ]
amova
blight
differentiation
dna
dna marker
fusarium
fusarium graminearum
genetic differentiation
genetic diversity
genetic markers
genetic variability
genetic variation
geographical variation
gibberella zeae
graminearum
island
kernels
marker
ontario
population
primer
prince edward island
quebec
random amplified polymorphic dna
rapd
strain differences
triticum aestivum
variability
variance

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