Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: CPP-23-83

[Submit comment/correction]

Reference	CPP-23-83
Title	Vegetative compatibility among Fusarium graminearum (Gibberella zeae) isolates from barley spikes in southern Manitoba
Journal	Canadian Journal of Plant Pathology
Year	2001
Volume	23
Pages	83-87
Author	McCallum BD Tekauz A Gilbert J
Abstract	Summary: Vegetative compatibility groups (VCGs) were determined for 43 Fusarium graminearum Schwabe (Gibberella zeae (Schwein.) Petch) isolates from barley using nitrate-nonutilizing mutants. Single isolates from 22 fields throughout southern Manitoba were used to evaluate regional diversity, and an additional 21 isolates from 10 spikes in 4 fields were used to estimate local diversity. Of 183 mutants generated, 67 utilized both nitrite and hypoxanthine (nit1), 89 utilized hypoxanthine but not nitrite (nit3), and 27 utilized nitrite but not hypoxanthine (NitM). A nit1 mutant from each isolate was paired on nitrate medium to either a nit3 or NitM mutant from all isolates. There were 21 VCGs among 22 isolates collected throughout Manitoba and 16 VCGs among 21 isolates used to estimate local diversity. When the 43 isolates were combined, 34 VCGs were identified. The average ratio of VCGs to isolates was 0.95 for isolates from different fields, 0.94 for isolates from different spikes within a field, and 0.90 for isolates within the same spike. The results indicate a high degree of VCG diversity in the F. graminearum population from barley spikes at the regional, local, and within-spike levels. This diversity should be considered when screening barley germplasm for fusarium head blight resistance
Keyword	[ Hide all but 1 of 33 ] aggressiveness barley blight compatibility diversity field field populations fusarium fusarium graminearum fusarium head blight fusarium head blight resistance germplasm gibberella gibberella zeae graminearum head blight manitoba media mutant nit mutants nitrate nitrate-nonutilizing mutants nitrite poae population resistance rye scab screening single spike vegetative compatibility vegetative compatibility groups

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.