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GrainGenes Reference Report: CPP-23-286

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Reference	CPP-23-286
Title	Molecular evidence for induction of phenylalanine ammonia-lyase during Puccinia graminis infection and elicitation in wheat
Journal	Canadian Journal of Plant Pathology
Year	2001
Volume	23
Pages	286-291
Author	Li HP Fischer R Liao YC
Abstract	Summary: Degenerate primers corresponding to the conserved sequences in phenylalanine ammonia-lyase (PAL) genes cloned from rice and parsley were used to amplify segments of the PAL genes from wheat (Triticum aestivum). More than 76% base sequence identity with the homologous genes confirmed that a 437 base pairs (bp) fragment was derived from the coding region of PAL. Using this fragment as probe, two PAL genes linked in tandem, Wpalt1 and Wpalt2, were isolated from a wheat genomic library. Northern blot and RNA dot blot analyses using a probe for Wpalt1 demonstrated that Wpalt1 expression was organ specific. Wpalt1 expression was high in roots and moderate in stems, but no detectable expression was found in leaves. Infection of leaves with Puccinia graminis f. sp. tritici (Pgt) induced, within 4 to 8 d, a high level of Wpalt1 transcripts in the Pgt resistant near-isogenic Prelude Sr6 line, while a delayed induction was observed in the Pgt susceptible isogenic Prelude sr6 line. In cell suspension cultures, treatment with chitin oligomers or an elicitor derived from P. graminis germ tube walls activated Wpalt1 gene expression. The Pgt-derived elicitor activated PAL expression earlier than did chitin oligomers, whereas chitin oligomers appeared to be more potent inducers of PAL expression than was the Pgt-derived elicitor
Gene	Sr6 (Triticum)
Keyword	[ Hide all but 1 of 57 ] canada cell cell culture cell suspension cultures chalcone synthase chitin culture degenerate primer differential induction elicitation elicitor f sp tritici fungal elicitor gene gene activation gene expression genomic library germ graminis high-level homologous genes hypersensitive resistance inducer induction infection l roots library molecular evidence northern oligomer pal parsley phenylalanine ammonia lyase phenylpropanoid metabolism plant development potent primer puccinia puccinia graminis region resistant rice root segment sequence stem stem rust stem rust fungus suspension suspension culture tandem transcript tritici triticum triticum aestivum tube wall

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