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GrainGenes Reference Report: BMG-10-578

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Reference	BMG-10-578
Title	DArT markers for the rye genome - genetic diversity and mapping
Journal	BMC Genomics
Year	2009
Volume	10
Pages	578
Author	Bolibok-Bragoszewska H [ Show all 6 ]
Abstract	ABSTRACT: BACKGROUND: Implementation of molecular breeding in rye (Secale cereale L.) improvement programs depends on the availability of high-density molecular linkage maps. However, the number of sequence-specific PCR-based markers available for the species is limited. Diversity Arrays Technology (DArT) is a microarray-based method allowing for detection of DNA polymorphism at several thousand loci in a single assay without relying on DNA sequence information. The objective of this study was the development and application of Diversity Arrays technology for rye. RESULTS: Using the PstI/TaqI method of complexity reduction we created a rye diversity panel from DNA of 16 rye varieties and 15 rye inbred lines, including parents of a mapping population consisting of 82 recombinant inbred lines. The usefulness of a wheat diversity panel for identification of DArT markers for rye was also demonstrated. We identified 1022 clones that were polymorphic in the genotyped ILs and varieties and 1965 clones that differentiated the parental lines L318 and L9 and segregated in the mapping population. Hierarchical clustering and ordination analysis were performed based on the 1022 DArT markers to reveal genetic relationships between the rye varieties and inbred lines included in the study. Chromosomal location of 1872 DArT markers was determined using wheat-rye addition lines and 1818 DArT markers (among them 1181 unique, non-cosegregating) were placed on a genetic linkage map of the cross L318 x L9, providing an average density of one unique marker every 2.68 cM. This is the most saturated rye linkage map based solely on transferable markers available at the moment, providing rye breeders and researches with a better choice of markers and a higher probability of finding polymorphic markers in the region of interest. CONCLUSIONS: The Diversity Arrays Technology can be efficiently and effectively used for rye genome analyses - assessment of genetic similarity and linkage mapping. The 11520-clone rye genotyping panel with several thousand markers with determined chromosomal location and accessible through an inexpensive genotyping service is a valuable resource for studies on rye genome organization and in molecular breeding of the species.
External Databases	http://dx.doi.org/10.1186/1471-2164-10-578
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DArT506995_0 DArT506995_1 DArT508915_0 DArT508915_1 DArT399950_0 DArT399950_1 DArT401486_0 DArT401486_1 DArT508148_0 DArT508148_1 DArT389583_0 DArT389583_1 DArT505461_0 DArT505461_1 DArT505845_0 DArT505845_1 DArT506997_0 DArT506997_1 DArT509301_0 DArT509301_1 DArT509685_0 DArT509685_1 DArT389584_0 DArT389584_1 DArT399568_0 DArT399568_1 DArT402640_0 DArT402640_1 DArT507382_0 DArT507382_1 DArT508918_0 DArT508918_1 DArT398801_0 DArT398801_1 DArT399569_0 DArT399569_1 DArT349456_0 DArT349456_1 DArT508151_0 DArT508151_1 DArT390738_0 DArT390738_1 DArT401106_0 DArT401106_1 DArT507000_0 DArT507000_1 DArT508152_0 DArT508152_1 DArT508536_0 DArT508536_1 DArT509688_0 DArT509688_1 DArT390355_0 DArT390355_1 DArT400339_0 DArT400339_1 DArT402259_0 DArT402259_1 DArT507001_0 DArT507001_1 DArT509689_0 DArT509689_1 DArT507770_0 DArT507770_1 DArT390358_0 DArT390358_1 DArT411094_0 DArT411094_1 DArT507388_0 DArT507388_1 DArT390359_0 DArT390359_1 DArT399959_0 DArT399959_1 DArT507006_0 DArT507006_1 DArT390361_0 DArT390361_1 DArT398809_0 DArT398809_1 DArT508543_0 DArT508543_1 DArT509695_0 DArT509695_1 DArT399578_0 DArT399578_1 DArT400346_0 DArT400346_1 DArT398811_0 DArT398811_1 DArT411483_0 DArT411483_1 DArT505857_0 DArT505857_1 DArT509313_0 DArT509313_1 DArT389596_0 DArT389596_1 DArT507010_0 DArT507010_1 DArT389597_0 DArT389597_1 DArT400349_0 DArT400349_1 DArT505475_0 DArT505475_1 DArT505859_0 DArT505859_1 DArT506243_0 DArT506243_1 DArT507395_0 DArT507395_1 DArT509315_0 DArT509315_1 DArT509699_0 DArT509699_1 DArT505860_0 DArT505860_1 DArT507012_0 DArT507012_1 DArT509316_0 DArT509316_1 DArT399967_0 DArT399967_1 DArT505861_0 DArT505861_1 DArT389984_0 DArT389984_1 DArT401888_0 DArT401888_1 DArT507398_0 DArT507398_1 DArT507399_0 DArT507399_1 DArT389602_0 DArT389602_1 DArT506632_0 DArT506632_1 DArT509320_0 DArT509320_1 DArT401891_0 DArT401891_1 DArT507017_0 DArT507017_1 DArT389988_0 DArT389988_1 DArT398820_0 DArT398820_1 DArT402276_0 DArT402276_1 DArT507018_0 DArT507018_1 DArT389989_0 DArT389989_1 DArT398821_0 DArT398821_1 DArT506251_0 DArT506251_1 DArT509323_0 DArT509323_1 DArT401894_0 DArT401894_1 DArT509324_0 DArT509324_1 DArT509708_0 DArT509708_1 DArT411111_0 DArT411111_1 DArT506637_0 DArT506637_1 DArT507405_0 DArT507405_1 DArT389992_0 DArT389992_1 DArT390376_0 DArT390376_1 DArT506254_0 DArT506254_1 DArT506638_0 DArT506638_1 DArT398825_0 DArT398825_1 DArT509711_0 DArT509711_1 DArT389610_0 DArT389610_1 DArT402282_0 DArT402282_1 DArT505488_0 DArT505488_1 DArT507408_0 DArT507408_1 DArT508944_0 DArT508944_1 DArT411499_0 DArT411499_1 DArT506641_0 DArT506641_1 DArT507793_0 DArT507793_1 DArT509329_0 DArT509329_1 DArT389612_0 DArT389612_1 DArT399596_0 DArT399596_1 DArT401516_0 DArT401516_1 DArT508178_0 DArT508178_1 DArT508946_0 DArT508946_1 DArT389997_0 DArT389997_1 DArT507795_0 DArT507795_1 DArT401902_0 DArT401902_1 DArT401135_0 DArT401135_1 DArT507029_0 DArT507029_1 DArT400752_0 DArT400752_1 DArT402672_0 DArT402672_1 DArT402288_0 DArT402288_1 DArT411504_0 DArT411504_1 DArT509718_0 DArT509718_1 DArT507415_0 DArT507415_1 DArT401906_0 DArT401906_1 DArT411122_0 DArT411122_1 DArT508184_0 DArT508184_1 DArT401139_0 DArT401139_1 DArT508569_0 DArT508569_1 DArT400756_0 DArT400756_1 DArT402676_0 DArT402676_1 DArT402292_0 DArT402292_1 DArT505882_0 DArT505882_1 DArT507034_0 DArT507034_1 DArT401909_0 DArT401909_1 DArT505499_0 DArT505499_1 DArT401526_0 DArT401526_1 DArT401143_0 DArT401143_1 DArT506653_0 DArT506653_1 DArT507037_0 DArT507037_1 DArT399608_0 DArT399608_1 DArT507422_0 DArT507422_1 DArT508190_0 DArT508190_1 DArT509726_0 DArT509726_1 DArT402298_0 DArT402298_1 DArT505888_0 DArT505888_1 DArT508192_0 DArT508192_1 DArT509728_0 DArT509728_1 DArT402299_0 DArT402299_1 DArT390780_0 DArT390780_1 DArT400764_0 DArT400764_1 DArT507426_0 DArT507426_1 DArT390397_0 DArT390397_1 DArT411517_0 DArT411517_1 DArT507427_0 DArT507427_1 DArT507811_0 DArT507811_1 DArT399614_0 DArT399614_1 DArT508196_0 DArT508196_1 DArT400767_0 DArT400767_1 DArT505893_0 DArT505893_1 DArT389632_0 DArT389632_1 DArT398848_0 DArT398848_1 DArT402305_0 DArT402305_1 DArT505511_0 DArT505511_1 DArT398850_0 DArT398850_1 DArT399618_0 DArT399618_1 DArT401538_0 DArT401538_1 DArT402306_0 DArT402306_1 DArT506664_0 DArT506664_1 DArT507816_0 DArT507816_1 DArT401539_0 DArT401539_1 DArT411523_0 DArT411523_1 DArT507817_0 DArT507817_1 DArT401156_0 DArT401156_1 DArT507818_0 DArT507818_1 DArT509738_0 DArT509738_1 DArT508203_0 DArT508203_1 DArT507436_0 DArT507436_1 DArT399626_0 DArT399626_1 DArT389264_0 DArT389264_1 DArT400016_0 DArT400016_1 DArT411152_0 DArT411152_1 DArT399251_0 DArT399251_1 DArT401939_0 DArT401939_1 DArT506687_0 DArT506687_1 DArT507072_0 DArT507072_1 DArT400412_0 DArT400412_1 DArT505540_0 DArT505540_1 DArT508233_0 DArT508233_1 DArT507851_0 DArT507851_1 DArT400041_0 DArT400041_1 DArT509008_0 DArT509008_1 DArT505940_0 DArT505940_1 DArT389295_0 DArT389295_1 DArT400432_0 DArT400432_1 DArT401584_0 DArT401584_1 DArT400049_0 DArT400049_1 DArT507095_0 DArT507095_1 DArT410806_0 DArT410806_1 DArT509404_0 DArT509404_1 DArT509405_0 DArT509405_1 DArT507871_0 DArT507871_1 DArT506336_0 DArT506336_1 DArT506725_0 DArT506725_1 DArT347242_0 DArT347242_1 DArT390470_0 DArT390470_1 DArT507116_0 DArT507116_1 DArT400457_0 DArT400457_1 DArT506753_0 DArT506753_1 DArT389724_0 DArT389724_1 DArT399709_0 DArT399709_1 DArT507525_0 DArT507525_1 DArT509061_0 DArT509061_1 DArT410848_0 DArT410848_1 DArT507149_0 DArT507149_1 DArT509455_0 DArT509455_1 DArT401263_0 DArT401263_1 DArT411247_0 DArT411247_1 DArT399348_0 DArT399348_1 DArT509467_0 DArT509467_1 DArT509468_0 DArT509468_1 DArT509086_0 DArT509086_1 DArT506786_0 DArT506786_1 DArT505256_0 DArT505256_1 DArT400900_0 DArT400900_1 DArT507180_0 DArT507180_1 DArT389385_0 DArT389385_1 DArT400524_0 DArT400524_1 DArT509106_0 DArT509106_1 DArT505269_0 DArT505269_1 DArT410896_0 DArT410896_1 DArT505273_0 DArT505273_1 DArT509115_0 DArT509115_1 DArT507197_0 DArT507197_1 DArT389784_0 DArT389784_1 DArT390169_0 DArT390169_1 DArT399769_0 DArT399769_1 DArT398626_0 DArT398626_1 DArT390180_0 DArT390180_1 DArT390566_0 DArT390566_1 DArT506831_0 DArT506831_1 DArT390577_0 DArT390577_1 DArT507224_0 DArT507224_1 DArT506078_0 DArT506078_1 DArT507621_0 DArT507621_1 DArT509160_0 DArT509160_1 DArT509161_0 DArT509161_1 DArT410948_0 DArT410948_1 DArT508394_0 DArT508394_1 DArT401733_0 DArT401733_1 DArT508785_0 DArT508785_1 DArT399441_0 DArT399441_1 DArT506487_0 DArT506487_1 DArT505724_0 DArT505724_1 DArT509565_0 DArT509565_1 DArT506883_0 DArT506883_1 DArT506502_0 DArT506502_1 DArT506121_0 DArT506121_1 DArT505740_0 DArT505740_1 DArT507662_0 DArT507662_1 DArT389865_0 DArT389865_1 DArT400236_0 DArT400236_1 DArT509202_0 DArT509202_1 DArT506131_0 DArT506131_1 DArT401006_0 DArT401006_1 DArT389489_0 DArT389489_1 DArT389490_0 DArT389490_1 DArT507290_0 DArT507290_1 DArT507680_0 DArT507680_1 DArT399869_0 DArT399869_1 DArT508841_0 DArT508841_1 DArT509225_0 DArT509225_1 DArT398726_0 DArT398726_1 DArT506927_0 DArT506927_1 DArT505394_0 DArT505394_1 DArT402574_0 DArT402574_1 DArT401041_0 DArT401041_1 DArT509623_0 DArT509623_1 DArT402194_0 DArT402194_1 DArT389523_0 DArT389523_1 DArT401815_0 DArT401815_1 DArT402199_0 DArT402199_1 DArT400288_0 DArT400288_1 DArT509254_0 DArT509254_1 DArT506569_0 DArT506569_1 DArT400295_0 DArT400295_1 DArT390701_0 DArT390701_1 DArT389936_0 DArT389936_1 DArT399920_0 DArT399920_1 DArT509658_0 DArT509658_1 DArT390325_0 DArT390325_1 DArT389564_0 DArT389564_1 DArT411452_0 DArT411452_1 DArT400706_0 DArT400706_1 DArT401092_0 DArT401092_1 DArT505834_0 DArT505834_1 DArT506219_0 DArT506219_1 DArT390734_0 DArT390734_1 DArT507380_0 DArT507380_1 DArT401108_0 DArT401108_1 DArT399576_0 DArT399576_1 DArT508159_0 DArT508159_1 DArT401115_0 DArT401115_1 DArT508930_0 DArT508930_1 DArT507779_0 DArT507779_1 DArT508932_0 DArT508932_1 DArT507014_0 DArT507014_1 DArT509319_0 DArT509319_1 DArT505865_0 DArT505865_1 DArT507019_0 DArT507019_1 DArT411495_0 DArT411495_1 DArT507025_0 DArT507025_1 DArT401132_0 DArT401132_1 DArT401903_0 DArT401903_1 DArT402673_0 DArT402673_1 DArT390003_0 DArT390003_1 DArT508955_0 DArT508955_1 DArT508189_0 DArT508189_1 DArT398846_0 DArT398846_1 DArT507044_0 DArT507044_1 DArT507435_0 DArT507435_1 DArT505920_0 DArT505920_1 DArT390070_0 DArT390070_1 DArT401641_0 DArT401641_1 DArT508307_0 DArT508307_1 DArT398596_0 DArT398596_1 DArT507959_0 DArT507959_1 DArT399388_0 DArT399388_1 DArT509515_0 DArT509515_1 DArT505687_0 DArT505687_1 DArT508380_0 DArT508380_1 DArT508381_0 DArT508381_1 DArT507231_0 DArT507231_1 DArT400572_0 DArT400572_1 DArT410953_0 DArT410953_1 DArT505342_0 DArT505342_1 DArT400991_0 DArT400991_1 DArT411049_0 DArT411049_1 DArT505823_0 DArT505823_1 DArT509282_0 DArT509282_1 DArT411072_0 DArT411072_1 DArT390349_0 DArT390349_1 DArT401883_0 DArT401883_1 DArT399580_0 DArT399580_1 DArT508163_0 DArT508163_1 DArT401523_0 DArT401523_1 DArT508579_0 DArT508579_1 DArT398481_0 DArT398481_1 DArT400039_0 DArT400039_1 DArT402345_0 DArT402345_1 DArT400434_0 DArT400434_1 DArT399677_0 DArT399677_1 DArT400083_0 DArT400083_1 DArT389347_0 DArT389347_1 DArT505280_0 DArT505280_1 DArT389788_0 DArT389788_1 DArT398620_0 DArT398620_1 DArT390620_0 DArT390620_1 DArT505743_0 DArT505743_1 DArT400652_0 DArT400652_1 DArT401036_0 DArT401036_1 DArT401100_0 DArT401100_1 DArT507008_0 DArT507008_1 DArT507048_0 DArT507048_1 DArT402312_0 DArT402312_1 DArT506458_0 DArT506458_1 DArT372662_0 DArT372662_1

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.