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GrainGenes Reference Report: BMG-18-567

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Reference	BMG-18-567
Title	Development of a high-density linkage map and mapping of the three-pistil gene (Pis1) in wheat using GBS markers
Journal	BMC Genomics
Year	2017
Volume	18
Pages	567-574
Author	Yang Z [ Show all 6 ]
Abstract	Background: The wheat mutant line three-pistil (TP) exhibits three pistils per floret. As TP normally has two or three seeds in each of the florets on the same spike, there is the possibility of increasing the number of grains per spike. Therefore, TP is a highly valuable mutant for breeding and for the study of floral development in wheat. To map the three-pistil gene (Pis1), genotyping-by-sequencing single-nucleotide polymorphism (GBS-SNP) data from an F2 mapping population (CM28 x CM28TP) was used to construct a genetic map that is of significant value. Results: In the present study, a high-density genetic map of wheat containing 2917 GBS-SNP markers was constructed. Twenty-one linkage groups were resolved, with a total length of 2371.40 cM. The individual chromosomes range from 2.64 cM to 454.55 cM with an average marker density of 0.81 cM. The Pis1 gene was mapped using this high-resolution map, and two flanking SNP markers tightly linked to the gene, M70 and M71, were identified. The Pis1 is 3.00 cM from M70 and 1.10 cM from M71. In bread wheat genome, M70 and M71 were found to delimit a physical distance of 3.40 Mb, which encompasses 127 protein-coding genes. To validate the GBS-generated genotypic data and to eliminate missing marker data in the Pis1 region, five Kompetitive Allele-Specific PCR (KASP) assays were designed from corresponding GBS sequences, which harbor SNPs that surround Pis1. Three KASP-SNP markers, KM70, KM71, and KM75, were remapped to the Pis1 gene region. Conclusions : This work not only lays the foundation for the map-based cloning of Pis1 but can also serve as a valuable tool for studying marker-trait association of important traits and marker-assisted breeding in wheat.
External Databases	https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-3960-7
Gene	Pis1 (Triticum) [ Show all 90 ]
Gene Class	Multi-gynoecium
Germplasm	Chuanmai 28 CM28TP
Locus	[ Hide all but 1 of 124 ] M70 KD550407 AAT06527 XP_008663459 XP_003576007 EMT00889 ABF50675 XP_010236956 EMT00293 EMS59123 XP_010230178 XP_010240336 EMS53270 EMS53269 EMT24888 EMT06562 XP_010239711 XP_015692361 ABA97240 EMS62021 AAS48876 ACG30677 EMT23289 EMT21921 EMT21701 EMS57067 EMS50970 EMT19251 CDM81269 XP_003563214 CAA77236 EMT11270 AAM90696 EMT02575 EMT24018 NP_001150854 YP_008239143 EMT32642 EMT01687 EMS60314 ADG43138 BAK04770 EMS61905 EMT21602 EMT32985 NP_001147064 EMT13358 EMS58119 EMT22445 EMT31378 CDX97861 EMT05261 CAH67649 CAE03004 EMT28400 EMS59176 AAM13439 NP_001136882 ABD37622 EMT02313 EMT06016 DAA60495 EMT14831 XP_007041489 EMT26270 EMT20109 EMT31390 EMS50969 BAF20825 EMT13733 EMT18108 EMT09554 EMS60315 EMS60316 CAH65993 XP_014750930 EMT28401 ACT22500 EMS55336 EMS51822 EMT08392 EMT03631 EMS51820 EMT03801 EMT09611 NP_001150358 BAB78487 NP_001148631 EAY80119 EMT27206 EMT02314 EMT22169 EMT22168 NP_001149849 EMS49112 EMT19210 EMT00056 EMS61907 EMT03164 EMT16430 EMT14107 EMT12050 EMT32641 EMT11405 CAH67057 EMT16310 CDJ26576 CDJ26577 AAL73394 EMT25260 BAS77745 DAA35664 EMS67906 CAJ86155 EMT13303 EMS65931 EMS52484 EMT11271 ACT32453 EMT32980 EMT30728 EMT24426 EMT14537 M71
Map Data	Wheat, CM28xCM28TP, Pis1 QTL
Probe	K2DL_CSv1_136805221 K2DL_CSv1_140258883
QTL	QPis1.CM28TP-2D
Keyword	Kompetitive allele-specific PCR (KASP) Three-pistil mutation Wheat Genotyping-by-sequencing (GBS)

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