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GrainGenes Reference Report: PBR-135-323

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Reference	PBR-135-323
Title	The art of attrition: development of robust oat microsatellites
Journal	Plant Breeding
Year	2016
Volume	135
Pages	323-334
Author	Dumlupinar Z [ Show all 12 ]
Abstract	Microsatellite or simple sequence repeat (SSR) markers are important tools for genetic analyses, especially those targeting diversity. The pri- mary objective of this study was to develop robust oat-based microsatel- lite markers from newly enriched genomic libraries to expand on a relatively small existing oat SSR toolbox. Microsatellite motifs characterized by (CA/GT), (AAT/TTA), (ATG/TAC) and (CATC/GTAG) repeats were targeted for enrichment. Preliminary screening showed that 90% of clones from the (CA/GT) and 79% of the clones from the (ATG/TAC) libraries contained repeats, while < 11% of the clones from (AAT/TTA) and (CATC/GTAG) libraries contained repeats. Subsequent sequencing of 1536 clones from the (CA/GT) and (ATG/TAC) libraries resulted in 539 and 578 SSRs for which primers were designed, respectively. A total of 246 SSRs were polymorphic across 11 oat lines. One hundred and twenty-five of the markers produced highly reproducible assays that interrogated 369 alleles at 193 loci. Of these, 79 robust assays interrogated 146 codominant alleles. These markers will be useful for a wide range of genetic analyses in oat including assessment of diversity and marker-assisted breeding.
External Databases	http://onlinelibrary.wiley.com/doi/10.1111/pbr.12362
Probe	[ Hide all but 1 of 126 ] AB_AM_910 AB_AM_911 AB_AM_912 AB_AM_913 AB_AM_914 AB_AM_915 AB_AM_59 AB_AM_65 AB_AM_66 AB_AM_68 AB_AM_69 AB_AM_70 AB_AM_73 AB_AM_74 AB_AM_76 AB_AM_77 AB_AM_79 AB_AM_81 AB_AM_83 AB_AM_87 AB_AM_93 AB_AM_95 AB_AM_108 AB_AM_111 AB_AM_123 AB_AM_124 AB_AM_130 AB_AM_155 AB_AM_183 AB_AM_185 AB_AM_202 AB_AM_219 AB_AM_223 AB_AM_226 AB_AM_227 AB_AM_232 AB_AM_238 AB_AM_242 AB_AM_258 AB_AM_259 AB_AM_260 AB_AM_269 AB_AM_275 AB_AM_283 AB_AM_285 AB_AM_290 AB_AM_299 AB_AM_303 AB_AM_322 AB_AM_323 AB_AM_324 AB_AM_327 AB_AM_329 AB_AM_340 AB_AM_342 AB_AM_349 AB_AM_354 AB_AM_361 AB_AM_397 AB_AM_399 AB_AM_425 AB_AM_431 AB_AM_439 AB_AM_451 AB_AM_453 AB_AM_457 AB_AM_467 AB_AM_469 AB_AM_483 AB_AM_485 AB_AM_488 AB_AM_491 AB_AM_493 AB_AM_494 AB_AM_512 AB_AM_541 AB_AM_550 AB_AM_553 AB_AM_576 AB_AM_577 AB_AM_603 AB_AM_680 AB_AM_688 AB_AM_689 AB_AM_692 AB_AM_709 AB_AM_805 AB_AM_814 AB_AM_824 AB_AM_825 AB_AM_829 AB_AM_831 AB_AM_833 AB_AM_839 AB_AM_842 AB_AM_844 AB_AM_845 AB_AM_846 AB_AM_847 AB_AM_851 AB_AM_853 AB_AM_854 AB_AM_858 AB_AM_863 AB_AM_873 AB_AM_874 AB_AM_878 AB_AM_888 AB_AM_897 AB_AM_905 AB_AM_906 AB_AM_907 AB_AM_908 AB_AM_916 AB_AM_956 AB_AM_993 AB_AM_994 AB_AM_1003 AB_AM_1013 AB_AM_1015 AB_AM_1024 AB_AM_1025 AB_AM_1043 AB_AM_1045 AB_AM_1052 AB_AM_1055

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