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GrainGenes Reference Report: PBR-135-323
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Reference
PBR-135-323

Title
The art of attrition: development of robust oat microsatellites

Journal
Plant Breeding

Year
2016

Volume
135

Pages
323-334

Author
[ Hide all but 1 of 12 ]
Dumlupinar Z
Brown R
Campbell R
Jellen EN
Anderson J
Bonman JM
Carson M
Chao SM
Obert D
Jackson E
Carson MC
Chao S

Abstract
Microsatellite or simple sequence repeat (SSR) markers are important tools for genetic analyses, especially those targeting diversity. The pri- mary objective of this study was to develop robust oat-based microsatel- lite markers from newly enriched genomic libraries to expand on a relatively small existing oat SSR toolbox. Microsatellite motifs characterized by (CA/GT), (AAT/TTA), (ATG/TAC) and (CATC/GTAG) repeats were targeted for enrichment. Preliminary screening showed that 90% of clones from the (CA/GT) and 79% of the clones from the (ATG/TAC) libraries contained repeats, while < 11% of the clones from (AAT/TTA) and (CATC/GTAG) libraries contained repeats. Subsequent sequencing of 1536 clones from the (CA/GT) and (ATG/TAC) libraries resulted in 539 and 578 SSRs for which primers were designed, respectively. A total of 246 SSRs were polymorphic across 11 oat lines. One hundred and twenty-five of the markers produced highly reproducible assays that interrogated 369 alleles at 193 loci. Of these, 79 robust assays interrogated 146 codominant alleles. These markers will be useful for a wide range of genetic analyses in oat including assessment of diversity and marker-assisted breeding.

External Databases
http://onlinelibrary.wiley.com/doi/10.1111/pbr.12362

Probe
AB_AM_910

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