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GrainGenes Reference Report: BLT-25-617

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Reference	BLT-25-617
Title	Cloning and expression of a new Tibetan hulless barley (Hordeum vulgare) beta-1,3-glucanase gene
Journal	Biotechnology Letters
Year	2003
Volume	25
Pages	617-622
Author	Yu Z Ying X Lin T Xiao-Lin Q Fang C
Abstract	Summary: A new full-length beta-1,3-glucanase cDNA was obtained by RT-PCR and RACE techniques from Tibet hulless barley and its complete gene sequence obtained by DNA Walking. Sequence alignment with the BLAST program showed that cDNA has high similarity with barley beta-1,3-glucanase II. The gene was functionally expressed in E. coli and the recombinant protein catalysed the hydrolysis of beta-1,3-glucan with an action pattern characteristic of a beta-1,3-glucan endohydrolase (EC 3.2.1.39). Southern blot analysis indicated that the gene is a member of a small gene family. RT-PCR and northern blot analysis indicated it is constitutively expressed in barley shoots
Keyword	[ Hide all but 1 of 31 ] (1->3)-beta-glucanase action pattern alignment barley beta-1,3-glucanase beta-1,3-glucanase gene biotechnology blast cdna cloning family gene gene family gene sequence hordeum hulless barley hydrolysis pathogenesis-related pattern program protein race recombinant protein rt-pcr sequence sequence alignment shoot similarities tibetan hulless barley transform walking

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