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GrainGenes Reference Report: BLT-24-721

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Reference	BLT-24-721
Title	Generation of transgenic wheat plants producing high levels of the osmoprotectant proline
Journal	Biotechnology Letters
Year	2002
Volume	24
Pages	721-725
Author	Sawahel WA Hassan AH
Abstract	Summary: Plasmid DNA (pBI-P5CS), containing the selectable neomycin phosphotransferase-II 'npt II' gene for kanamycin resistance and the reporter beta-glucuronidase 'gus' gene as well as the Vigna aconitifolia delta(1)-pyrroline-5-carboxylate synthetase 'P5CS' cDNA that encodes enzymes required for the biosynthesis of proline, was delivered into wheat plants using Agrobacterium-mediated gene transfer via indirect pollen system. Southern, northern and western blot analysis demonstrated that the foreign gene had been transferred, expressed and integrated into wheat chromosomal DNA. Salinity test indicated that proline acts as an osmoprotectant and its overproduction in transgenic wheat plants results in the increased tolerance to salt
Keyword	[ Hide all but 1 of 39 ] agrobacterium-mediated transformation donor epistatic interaction f-2 population field field conditions fine mapping function gene genetic background hd2 heading heading date indica japanese japonica japonica rice linkage map locus marker marker-assisted selection mendelian factors nearly isogenic line oryza sativa population progeny qtl quantitative trait quantitative trait loci quantitative trait locus recurrent rflp rflp marker rice segment selection short day target trait

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