Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: ZUM-3-15

[Submit comment/correction]

Reference	ZUM-3-15
Title	Molecular detection of phytoplasmas in oats, barley, and Triticosecale and their classification based on 16S rRNA gene polymorphisms
Journal	Zemes ukio Mokslai
Year	2004
Volume	3
Pages	15-19
Author	Urbanaviciene L Jomantiene R Valiunas D Davis RE
Abstract	Diseased plants of oats ( Avena sativa ), barley ( Hordeum vulgare ) and Triticosecale ( Triticum x Secale ) were observed in the fields in the Vilnius region of Lithuania. Disease symptoms exhibited by the plants included yellowing of leaves, general stunting, sterility and deformation of spikes, dwarfed spikes, and twisted awns, indicating possible infection by phytoplasmas. A phytoplasma-characteristic fragment of 16S rDNA was amplified in nested polymerase chain reactions which were primed by phytoplasma universal primers, confirming phytoplasma infection in the symptomatic plants. Restriction fragment length polymorphism analysis of amplified 16S rDNA indicated that the diseased oats were infected by the group 16SrI (aster yellows, AY, group) phytoplasma strain belonging to the subgroup 16SrI-L, and that barley and Triticosecale were infected by strains belonging to the subgroup 16SrI-B. The phytoplasma strains found in oat, barley and Triticosecale were named oat yellows (OatY), barley deformation (BaDef), and Triticosecale stunt (TrSt) phytoplasmas, respectively. This is a first reported characterization of AY phytoplasmas in cereal crops of barley and Triticosecale
Keyword	[ Hide all but 1 of 18 ] awns barley classification genetic marker genetic polymorphism infection oat plant diseases plant pathogenic bacteria plant pathogens polymerase chain reaction restriction fragment length polymorphism rflp ribosomal dna spikes sterility symptoms triticale

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.