GrainGenes Reference Report: XCU-02-155
Reference
XCU-02-155
Title
Introgression of barley chromatin into wheat genome through chromosome engineering
Contained in
(2002) Current Topics in Plant Biology, Volume 3.
Year
2002
Pages
155-165
Author
Murai K Taketa S
Abstract
Barley (Hordeum vulgare) is a potential new source of genes for wheat (Triticum aestivum) improvement. We previously reported that barley chromosome 5H has genes for early-heading which function in the wheat genome. To introduce the barley genes for early-heading on chromosome 5H into wheat, we are producing wheat-barley 5H chromosome recombinant lines using 5B nullisomy and molecular markers. For the effective screening of recombinants involving 5H, we developed 18 barley-allele specific PCR markers (amplicons) which can be distinguished from their wheat counterparts without restriction enzyme digestions for chromosome 5H. Among the progenies of the line with nullisomic-5B trisomic-5A monosomic-5H (19II+5AIII+5HI) lacking the Ph1 gene on chromosome 5B, we were able to detect several plants having putative wheat-barley recombinant chromosomes by using 5H allele-specific amplicon markers
Keyword
[ Hide all but 1 of 11 ] barley chromatin chromosome gene transfer genetic engineering genetic marker genetic transformation introgression progeny screening transgenic plants
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: XCU-02-155
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||