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GrainGenes Reference Report: TPJ-39-206

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Reference	TPJ-39-206
Title	The calcium-dependent protein kinase HvCDPK1 mediates the gibberellic acid response of the barley aleurone through regulation of vacuolar function
Journal	Plant Journal
Year	2004
Volume	39
Pages	206-218
Author	McCubbin AG Ritchie SM Swanson SJ Gilroy S
Abstract	Summary: In the aleurone cells of the cereal grain, gibberellic acid (GA) induces the secretion of hydrolases that mobilize endosperm reserves to fuel early seedling growth. GA is known to trigger a range of cellular responses, including increases in cytoplasmic calcium, vacuolar reserve mobilization, gene transcription, and the synthesis and secretion of hydrolases. To further define elements of the Ca2+-dependent GA response machinery, we have cloned a Ca2+-dependent protein kinase (HvCDPK1) from these cells. Although expression of an inactivated (D140N) version of this kinase did not affect GA-induced gene expression or changes in cytosolic Ca2+, it did inhibit secretion, cell vacuolation, and vacuolar acidification, all responses linked to the GA response. Additionally, recombinant wild-type HvCDPK1 activated the V-type H+-ATPase present in isolated aleurone vacuoles. These results suggest HvCDPK1 may mediate Ca2+-dependent events of the GA response, such as control of vacuolar function, that lie downstream of transcriptional regulation
External Databases	Pubmed: 15225286
Keyword	[ Hide all but 1 of 41 ] aleurone aleurone cells alpha-amylase barley barley aleurone ca2+ calcium cdpk cell cereal grain control cytoplasmic calcium cytosolic ca2+ element endoplasmic reticulum endosperm ga ga response gene growth h+-atpase hordeum vulgare hydrolases ion-channel kinase machinery messenger rna protein protoplast recombinant regulation responses secretion seed development seedling seedling growth signal transduction transcription transcriptional regulation vacuole wild-type

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