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GrainGenes Reference Report: TPJ-34-39

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Reference	TPJ-34-39
Title	Increased dehydrin promoter activity caused by HvSPY is independent of the ABA response pathway
Journal	Plant Journal
Year	2003
Volume	34
Pages	39-46
Author	Robertson M
Abstract	Summary: A barley SPINDLY protein, HvSPY, is a negative regulator of gibberellin (GA) action. It is also found to be a positive regulator of the promoter of a barley dehydrin (Dhn) gene which is abscisic acid (ABA) upregulated. To investigate whether HvSPY acts through the ABA signaling pathway to upregulate the Dhn promoter, functional characterization was carried out by co-bombardment experiments. These experiments used Dhn promoter-GUS reporter constructs and an effector construct to overexpress HvSPY protein in barley aleurone. ABA dose-response experiments with and without HvSPY overexpression showed that the induction by HvSPY occurred in addition to the ABA effect. Gibberellic acid (GA3) did not reduce the induction by ABA, but it had a small, although significant, effect on the ability of HvSPY to upregulate. The induction of promoter activity of Dhn by HvSPY required the intact protein, and a small deletion in the tetratricopeptide repeat (TPR) region reduced this ability significantly. When a promoter region containing an element for ABA responsiveness was mutagenized or deleted, the mutant promoters lost ABA responsiveness but remained responsive to HvSPY. In addition, HvSPY did not increase promoter activities of other ABA-upregulated genes. Taken together, these results indicate that HvSPY and ABA both regulate promoter activity of Dhn, and that HvSPY acts independently of the ABA signaling pathway
External Databases	Pubmed: 12662307
Keyword	[ Hide all but 1 of 36 ] aba aba signaling abre acid aleurone barley barley aleurone barley spindly dehydrin deletion element functional-characterization gene gibberellin gibberellin signal-transduction glcnac transferase hordeum vulgare l. increase induction maize mutant negative regulator overexpression pathway promoter promoter activity protein region regulator repeat reporter response pathway responsiveness sequence analysis signaling signaling pathway

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