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GrainGenes Reference Report: TPJ-33-1001

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Reference	TPJ-33-1001
Title	Correlated clustering and virtual display of gene expression patterns in the wheat life cycle by large-scale statistical analyses of expressed sequence tags
Journal	Plant Journal
Year	2003
Volume	33
Pages	1001-1011
Author	Ogihara Y [ Show all 7 ]
Abstract	Summary: Compared to rice, wheat exhibits characteristic growth habits and contains complex genome constituents. To assess global changes in gene expression patterns in the wheat life cycle, we conducted large-scale analysis of expressed sequence tags (ESTs) in common wheat. Ten wheat tissues were used to construct cDNA libraries: crown and root from 14-day-old seedlings; spikelet from early and late flowering stages; spike at the booting stage, heading date and flowering date; pistil at the heading date; and seeds at 10 and 30 days post-anthesis. Several thousand colonies were randomly selected from each of these 10 cDNA libraries and sequenced from both 5' and 3' ends. Consequently, a total of 116 232 sequences were accumulated and classified into 25 971 contigs based on sequence homology. By computing abundantly expressed ESTs, correlated expression patterns of genes across the tissues were identified. Furthermore, relationships of gene expression profiles among the 10 wheat tissues were inferred from global gene expression patterns. Genes with similar functions were grouped with one another by clustering gene expression profiles. This technique might enable estimation of the functions of anonymous genes. Multidimensional analysis of EST data that is analogous to the microarray experiments may offer new approaches to functional genomics of plants
External Databases	Pubmed: 12631325
Keyword	[ Hide all but 1 of 47 ] arabidopsis cdna cdna library cdna microarray chromosomal location common wheat complexes contig correlation clustering of expression patterns cycle draft sequence est estimation expression pattern flowering flowering date functional genomics gene gene expression gene search in wheat genome genomic genomics growth growth habit habit heading heading date hexaploid wheat homology large-scale est analysis life molecular characterization postanthesis promoter activity protein kinase rice rice genome root seed seedling sequence sequence tags tissue translation initiation triticum aestivum virtual display

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