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GrainGenes Reference Report: TPJ-33-707

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Reference	TPJ-33-707
Title	A Mak-like kinase is a repressor of GAMYB in barley aleurone
Journal	Plant Journal
Year	2003
Volume	33
Pages	707-717
Author	Woodger FJ Gubler F Pogson BJ Jacobsen JV
Abstract	Summary: GAMYB is a gibberellin (GA)-regulated activator of hydrolase gene expression in the aleurone layer of germinating cereal grains. Although it is clear that GAMYB expression is regulated by GA, more remains to be understood about how this transcription factor operates within the GA-response pathway. In order to isolate new components from the GA-response pathway, barley aleurone libraries were screened for GAMYB-binding proteins using a recently developed yeast two-hybrid system, which is compatible with the use of transcription factors as baits. We isolated a new member of the emerging Mak-subgroup of cdc2- and MAP kinase-related protein kinases. We have termed this GAMYB-binding protein KGM (for kinase associated with GAMYB). Transient expression of KGM specifically repressed alpha-amylase promoter activity at the level of GAMYB function but a mutation designed to de-stabilise the activation loop of KGM alleviated this repression. We propose that KGM is a negative regulator of GAMYB function in aleurone that may prevent precocious hydrolase gene expression
External Databases	Pubmed: 12609043
Keyword	[ Hide all but 1 of 38 ] abscisic acid activation activator aleurone aleurone layer alpha-subunit barley barley aleurone cereal aleurone cereal grains dna binding gamyb gene gibberellin gibberellin signal-transduction grains heterotrimeric g-protein hormonal regulation isolate layer mak map mutation negative regulator pathway promoter protein protein kinase protein-protein interactions regulator repression signalling transcription transcription factor transcription factor bpbf transient transient expression yeast

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