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GrainGenes Reference Report: TPJ-31-223

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Reference	TPJ-31-223
Title	Constructing plant radiation hybrid panels
Journal	Plant Journal
Year	2002
Volume	31
Pages	223-228
Author	Wardrop J Snape J Powell W Machray GC
Abstract	Summary: Radiation hybrid (RH) mapping, a somatic cell genetic technique, has been developed in animal systems as a general approach for the construction of long-range physical maps of chromosomes. This statistical method relies on X-ray induced breakage of chromosomes to determine the physical distance between markers, as well as their order on the chromosome. The method can be applied to single chromosomes or across the whole genome. The generation of plant (barley) radiation hybrids and their culture in vitro is described here. PCR-based marker systems are used to verify hybrid status and to demonstrate genome coverage. RH panels of the type generated can be used for physical mapping, map-based cloning, or sequence contig assembly. RH resources will greatly aid the physical characterisation of crop plants with large genomes
Keyword	[ Hide all but 1 of 29 ] aid assembly barley breakage cell cell fusion cloning contig crop plants distance generation genetic marker human genome in vitro map map-based cloning mapping method mouse genome order pcr-based marker physical map physical mapping plant genome protoplast radiation sequence vitro whole

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